Recent studies from our laboratory have shown that alcohol and burn injury impair intestinal barrier and immune functions. Although multiple factors can contribute to impaired intestinal barrier function, such alteration could result from a decrease in intestinal blood flow and oxygen delivery. Therefore in this study we tested the hypothesis that alcohol ingestion before burn injury reduces splanchnic blood flow and oxygen delivery. Rats (250 g) were gavaged with alcohol to achieve a blood EtOH level in the range of 100 mg/dL before burn or sham injury (25% TBSA). Twenty four hours after injury, animals were anesthetized with methoxyflurane. Blood pressure, cardiac output (CO), +/-dp/dt, organ blood flow (BF; ml/min/100g) and oxygen delivery (DO₂; mg/ml/100g) were determined. Cardiac output and organ blood flow were determined by using a radioactive microsphere technique. Our results indicate that blood pressure, CO and +dp/dt was decreased in rats receiving combined insult of alcohol and burn injury compared to the rats receiving either burn injury or alcohol alone. This is accompanied by a decrease in BF and DO₂ to liver and intestine. No significant change in blood flow to the coronary arteries (heart), brain, lung, skin and muscles was observed following alcohol and burn injury. In conclusion, results presented here suggest that alcohol ingestion before burn injury reduces splanchnic blood flow and oxygen delivery. Such decreases in BF and DO₂ may cause hypoxic insult to intestine and liver. While a hypoxic insult to liver would result in release of pro-inflammatory mediators, a similar insult to intestine will likely perturb both intestinal immune cell and barrier functions as observed in our previous study.