Hemodynamic analysis was conducted to get uncertainty in clinical measurements of coronary flow reserve (CFR) and fractional flow reserve (FFR) over the patho-physiological conditions in a patient group with coronary artery disease during angioplasty. The vasodilation-distal perfusion pressure curve (CFR-p_rh) was obtained for two guidewire sizes 0.35 mm and 0.46 mm. Our hypothesis is that a guidewire spanning the lesions elevates the pressure gradient and reduces the flow during hyperemic measurements. The patient group maximal CFR-p_rh was uniquely determined by the intersections of measured values of CFR and calculated values of p_rh for the native and residual epicardial, without microvascular disease, lesions during angioplasty. Extrapolation of the linear curve gave a zero-coronary flow mean pressure p_zf of ~20 mmHg and, corresponding value of p_rh of 55 mmHg in the native lesions that coincided with the level that causes ischemia in human hearts. On this linear curve, values of CFR and FFR_myo (under patho-physiological condition), and CFR_g and FFR_myog (in the presence of guidewire) were obtained in native and residual lesions. A strong linear correlation was found between CFR and CFR_g (0.46 mm:CFR = CFR_gx0.689 + 1.271 (R²=0.99); 0.35 mm:CFR = CFR_gx0.757 + 1.004 (R²=0.99)), and between FFR_myo and FFR_myog (0.46 mm:FFR_myo = FFR_myogx0.737 + 0.263 (R²=0.99); 0.35 mm:FFR_myo = FFR_myogx0.790 + 0.210 (R²=0.99)). This study establishes a strong correlation between CFR and CFR_g and, between FFR_myo and FFR_myog which could be used to obtain the true state of occlusion in the coronary artery during angioplasty.