| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology and Pharmacology, West Virginia University, Morgantown, WV, USA
2 Department of Pathology and Physiology Branch, NIOSH HELD, Morgantown, WVU, USA
* To whom correspondence should be addressed. E-mail: phe{at}hsc.wvu.edu.
Our previous study demonstrated that the firm attachment of leukocytes to microvessel walls does not necessarily increase microvessel permeability (Am J Pysiol 283: H2420, 2002). To further understand the mechanisms of the permeability increase associated with leukocyte accumulation during acute inflammation, we investigated the direct relationship of the reactive oxygen species (ROS) release during neutrophil respiratory burst to the changes in microvessel permeability and endothelial [Ca2+]i in intact microvessels. ROS release from activated neutrophils was quantified by measuring changes in chemiluminescence (CL). When isolated rat neutrophils (2 x 106/ml) were exposed to formyl-Met-Leu-Phe-OH (fMLP, 10 µM), CL transiently increased from 1.2 ± 0.2 x 104to a peak value of 6.7 ± 1.0 x 104 cpm/min (n=12). Correlatively, perfusing individual microvessels with fMLP-stimulated neutrophils in suspension (2 x 107/ml) increased hydraulic conductivity, Lp, to 3.7 ± 0.4 times the control value (n=5) and increased endothelial [Ca2+]i from 84 ± 7 nM to a mean peak value of 170 ± 7 nM. In contrast, perfusing vessels with fMLP alone did not affect basal Lp. The application of antioxidant agents, superoxide dismutase (SOD), vitamin C, or an iron chelator, deferoxamine mesylate (DFO), attenuated the ROS release in fMLP-stimulated neutrophils and abolished the increases in Lp. These results indicate that the release of ROS from fMLP-stimulated neutrophils increases microvessel permeability and endothelial [Ca2+]i independently from leukocyte adhesion and migration process.
This article has been cited by other articles:
|
|
 |
|
  Y. Jiang, K. Wen, X. Zhou, D. Schwegler-Berry, V. Castranova, and P. He Three-dimensional localization and quantification of PAF-induced gap formation in intact venular microvessels Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H898 - H906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Bertram, S. Baltic, N. L. Misso, K. D. Bhoola, P. S. Foster, P. J. Thompson, and M. Fogel-Petrovic Expression of kinin B1 and B2 receptors in immature, monocyte-derived dendritic cells and bradykinin-mediated increase in intracellular Ca2+ and cell migration J. Leukoc. Biol., June 1, 2007; 81(6): 1445 - 1454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. He, H. Zhang, L. Zhu, Y. Jiang, and X. Zhou Leukocyte-platelet aggregate adhesion and vascular permeability in intact microvessels: role of activated endothelial cells Am J Physiol Heart Circ Physiol, August 1, 2006; 291(2): H591 - H599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H. Sarelius, J. M. Kuebel, J. Wang, and V. H. Huxley Macromolecule permeability of in situ and excised rodent skeletal muscle arterioles and venules Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H474 - H480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhu and P. He fMLP-stimulated release of reactive oxygen species from adherent leukocytes increases microvessel permeability Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H365 - H372. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
