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1 Farmacologia, Universidad Autonoma de Madrid, Madrid, Madrid, Spain
2 Fisiologia, Universidad Autonoma de Madrid, Madrid, Madrid, Spain
3 Fisiologia, Universidad de Sao Paulo, Sao Paulo, Sao Paulo, Brazil
4 Farmacologia, Universidad Autonoma de Madrid, Madrid, Madrid, Spain; Ciencias de la Salud, Universidad Rey Juan Carlos, Alcorcon, Madrid, Spain
* To whom correspondence should be addressed. E-mail: mercedes.salaices{at}uam.es.
We have previously described that chronic administration of ouabain induces hypertension and functional alterations in mesenteric resistance arteries (MRA). The aim of this study was to analyse if ouabain treatment also alters the structural and mechanical properties of MRA. Wistar rats were treated for 5 weeks with ouabain (8.0 µg/day s.c.). Vascular structure and mechanics of the third order branches of the mesenteric artery were assessed with pressure myography and confocal microscopy. Total collagen content was determined by picrosirius red staining, collagen I/III was analyzed by Western blot and elastin was studied by confocal microscopy. Vascular reactivity was analyzed by wire myography. Internal and external diameters and cross-sectional area were diminished whereas wall:lumen ratio was increased in arteries from ouabain-treated rats compared to controls. In addition, arteries from ouabain-treated rats were stiffer. Ouabain treatment decreased smooth muscle cell number and increased total and I/III collagens in the vascular wall. However, this treatment did not modify adventitia and media thickness, nuclei morphology, elastin structure and vascular reactivity to noradrenaline and acetylcholine. The present work shows hypotrophic inward remodeling of mesenteric resistance arteries from ouabain-treated rats that seems to be consequence of combination of decreased cell number and impaired distension of the artery, possibly due to a higher stiffness associated to collagen deposition. The narrowing of resistance arteries could play a role in the pathogenesis of hypertension in this model.
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