| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Cytomorphology, University of Cagliari School of Medicine, Cagliari, Italy
2 Department of Oral Diagnosis, Case Western Reserve University School of Dental Medicine, Cleveland, OH, USA
3 Medical Research Service (151W), Loius Stokes VA Medical Center, Cleveland, OH, USA
* To whom correspondence should be addressed. E-mail: charles.hoppel{at}case.edu.
To determine if there are structural differences in two topologically separated, biochemically-defined mitochondrial populations in rat heart myocytes, the interior of these organelles was examined by high resolution scanning electron microscopy. Based on a count of 159 in situ subsarcolemmal mitochondria (SSM) (those that directly abut the sarcolemma), these organelles possess mainly lamelliform cristae (77%), whereas the cristae in in situ interfibrillar mitochondria (IFM) (those situated between the myofibrils) (300 counted) are mainly tubular (55%) or a mixture of tubular and lamelliform (24%). Isolated SSM (374 counted), like their in situ counterparts, have predominantly lamelliform cristae (75%). The proportions of crista types in isolated IFM (337 counted) have been altered, with only 20% of these organelles retaining exclusively tubular cristae, whereas 58% are mixed-- of the latter, lamelliform cristae predominate. This finding suggests that in contrast to SSM, the cristae in IFM are structurally plastic, changing during isolation. These observations on more than a thousand organelles provide the first quantitative morphological evidence for definitive differences between the two populations of cardiac mitochondria.
This article has been cited by other articles:
|
|
 |
|
  L. C. Heather, C. A. Carr, D. J. Stuckey, S. Pope, K. J. Morten, E. E. Carter, L. M. Edwards, and K. Clarke Critical role of complex III in the early metabolic changes following myocardial infarction Cardiovasc Res, August 31, 2009; (2009) cvp276v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Kavazis, S. Alvarez, E. Talbert, Y. Lee, and S. K. Powers Exercise training induces a cardioprotective phenotype and alterations in cardiac subsarcolemmal and intermyofibrillar mitochondrial proteins Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H144 - H152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. R. Dabkowski, C. L. Williamson, V. C. Bukowski, R. S. Chapman, S. S. Leonard, C. J. Peer, P. S. Callery, and J. M. Hollander Diabetic cardiomyopathy-associated dysfunction in spatially distinct mitochondrial subpopulations Am J Physiol Heart Circ Physiol, February 1, 2009; 296(2): H359 - H369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Kavazis, J. M. McClung, D. A. Hood, and S. K. Powers Exercise induces a cardiac mitochondrial phenotype that resists apoptotic stimuli Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H928 - H935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Johnson, R. A. Harris, S. French, P. V. Blair, J. You, K. G. Bemis, M. Wang, and R. S. Balaban Tissue heterogeneity of the mammalian mitochondrial proteome Am J Physiol Cell Physiol, February 1, 2007; 292(2): C689 - C697. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
