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1 Department of Cytomorphology, University of Cagliari School of Medicine, Cagliari, Italy
2 Department of Oral Diagnosis, Case Western Reserve University School of Dental Medicine, Cleveland, OH, USA
3 Medical Research Service (151W), Loius Stokes VA Medical Center, Cleveland, OH, USA
* To whom correspondence should be addressed. E-mail: charles.hoppel{at}case.edu.
To determine if there are structural differences in two topologically separated, biochemically-defined mitochondrial populations in rat heart myocytes, the interior of these organelles was examined by high resolution scanning electron microscopy. Based on a count of 159 in situ subsarcolemmal mitochondria (SSM) (those that directly abut the sarcolemma), these organelles possess mainly lamelliform cristae (77%), whereas the cristae in in situ interfibrillar mitochondria (IFM) (those situated between the myofibrils) (300 counted) are mainly tubular (55%) or a mixture of tubular and lamelliform (24%). Isolated SSM (374 counted), like their in situ counterparts, have predominantly lamelliform cristae (75%). The proportions of crista types in isolated IFM (337 counted) have been altered, with only 20% of these organelles retaining exclusively tubular cristae, whereas 58% are mixed-- of the latter, lamelliform cristae predominate. This finding suggests that in contrast to SSM, the cristae in IFM are structurally plastic, changing during isolation. These observations on more than a thousand organelles provide the first quantitative morphological evidence for definitive differences between the two populations of cardiac mitochondria.
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