| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print January 3, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00875.2001
Submitted on October 10, 2001
Accepted on December 21, 2001
1 Medicine, University of Rochester Medical Center, Rochester, New York, USA
2 Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
3 Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin, USA
4 Human Cancer Genetics, Ohio State University, Columbus, Ohio, USA
5 Lawrence Livermore National Laboratory, Livermore, California, USA
* To whom correspondence should be addressed. E-mail: joseph_miano{at}urmc.rochester.edu.
Defining regulatory elements governing cell-restricted gene expression can be difficult since cis-elements may reside tens of kilobases away from start sites of transcription. Artificial chromosomes, which harbor hundreds of kilobases of genomic DNA, preserve a large sequence landscape containing most, if not all, regulatory elements controlling the expression of a particular gene. Here, we report on the use of a bacterial artificial chromosome (BAC) to begin understanding the in vivo regulation of smooth muscle calponin (SM-Calp). Long and accurate polymerase chain reaction (PCR), sequencing, and in silico analyses facilitated the complete sequence annotation of a BAC harboring human SM-Calp (hSM-Calp). RNase protection, in situ hybridization, Western blotting, and immunohistochemistry assays showed the BAC clone faithfully expressed hSM-Calp in both cultured cells and transgenic mice. Moreover, expression of hSM-Calp mirrored that of endogenous mouse SM-Calp suggesting that all cis-regulatory elements governing hSM-Calp expression in vivo were contained within the BAC. These BAC mice represent a new model system in which to systematically assess regulatory elements governing SM-Calp transcription in vivo.
This article has been cited by other articles:
|
|
 |
|
  X. Long, R. D. Bell, W. T. Gerthoffer, B. V. Zlokovic, and J. M. Miano Myocardin Is Sufficient for a Smooth Muscle-Like Contractile Phenotype Arterioscler Thromb Vasc Biol, August 1, 2008; 28(8): 1505 - 1510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Nagel, T. Aizawa, K.-I. Jeon, W. Liu, A. Mohan, H. Wei, J. M. Miano, V. A. Florio, P. Gao, V. A. Korshunov, et al. Role of Nuclear Ca2+/Calmodulin-Stimulated Phosphodiesterase 1A in Vascular Smooth Muscle Cell Growth and Survival Circ. Res., March 31, 2006; 98(6): 777 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Xu, Y.-S. Ho, R. P. Ritchie, and L. Li Human SM22alpha BAC encompasses regulatory sequences for expression in vascular and visceral smooth muscles at fetal and adult stages Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1398 - H1407. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
