During exercise, reflex renal vasoconstriction helps to maintain blood pressure and redistributes blood flow to the contracting muscle. Prior work has shown that gender and muscle mass can influence certain cardiovascular responses to exercise. Whether gender and/or muscle mass influence renal vasoconstrictor responses to exercise is unknown. We studied age and body mass index (BMI) matched healthy men (n = 10) and women (n = 10) during handgrip (HG, small muscle mass) and quadriceps contraction (QC, large muscle mass) as beat-to-beat changes in renal blood flow velocity (RBV; Duplex Ultrasound), mean arterial pressure (MAP; Finapres) and heart rate (ECG) were monitored. Renal vascular resistance index (RVR) was calculated by dividing MAP by RBV. Responses to HG vs. QC were compared in 13 subjects. We found that: 1) RVR responses to short bouts (15 sec) and fatiguing HG were similar in men and women (% change in RVR during 15 sec HG at 70% maximum voluntary contraction [MVC], male 23 ± 4% vs. female 31 ± 4%, P = NS); 2) post-handgrip circulatory responses were also similar in men and women; and 3) HG and QC were similar during short bouts (15 sec; % change in RVR during HG at 50% MVC, arm 19 ± 3% vs. leg 18 ± 5%, P = NS). Our findings suggest that muscle reflex mediated renal vasoconstriction is similar in men and women during static exercise. Moreover, muscle mass does not contribute to the magnitude of the reflex renal vasoconstrictor response seen with muscle contraction.