AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (January 3, 2002). doi:10.1152/ajpheart.00938.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/H1665    most recent
00938.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by VanBuren, P.
Right arrow Articles by Alpert, N. R
Right arrow Search for Related Content
PubMed
Right arrow Articles by VanBuren, P.
Right arrow Articles by Alpert, N. R

Articles in PresS, published online ahead of print January 3, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00938.2001
Submitted on October 30, 2001
Accepted on December 24, 2001

Cardiac Troponin T Isoforms Demonstrate Similar Effects on Mechanical Performance in a Regulated Contractile System

Peter VanBuren1*, Shari L Alix2, Joseph A Gorga2, Kelly J Begin2, Martin M LeWinter2, and Norman R Alpert3

1 Cardiac Unit, Department of Medicine, University of Vermont, Burlington, Vermont, USA; Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont, USA
2 Cardiac Unit, Department of Medicine, University of Vermont, Burlington, Vermont, USA
3 Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont, USA

* To whom correspondence should be addressed. E-mail: vanburen{at}physiology.med.uvm.edu.

Alteration of troponin T ( TnT ) isoform expression has been reported in human and animal models of myocardial failure. The two adult beef cardiac TnT isoforms were isolated for comparative functional analysis. Thin filaments were reconstituted containing pure populations of the isoforms. The in vitro motility assay was used to directly compare the effect of the two TnT isoforms on force and unloaded shortening as a function of free calcium. We found no significant differences between the two isoforms in terms of calcium sensitivity, cooperativity, or maximal activation (velocity and force) as assessed in a fully calcium regulated system. Activation by myosin strong binding was similar for thin filaments containing either of the two TnT isoforms. While maximally activated velocity and cooperativity was depressed at pH 6.5, no difference between thin filaments containing the two isoforms was detect. In light of the small magnitude of the TnT isoform shifts detected in myocardial failure, and the lack of significant mechanical effect detected in the motility assay, variable TnT isoform expression is unlikely to be any functional significance in heart failure.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
Y. Okada, M. J. Toth, and P. VanBuren
Skeletal muscle contractile protein function is preserved in human heart failure
J Appl Physiol, April 1, 2008; 104(4): 952 - 957.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. J. Belin, M. P. Sumandea, T. Kobayashi, L. A. Walker, V. L. Rundell, D. Urboniene, M. Yuzhakova, S. H. Ruch, D. L. Geenen, R. J. Solaro, et al.
Left ventricular myofilament dysfunction in rat experimental hypertrophy and congestive heart failure
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2344 - H2353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. Nassar, N. N. Malouf, L. Mao, H. A. Rockman, A. E. Oakeley, J. R. Frye, J. R. Herlong, S. P. Sanders, and P. A. W. Anderson
cTnT1, a cardiac troponin T isoform, decreases myofilament tension and affects the left ventricular pressure waveform
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1147 - H1156.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. M. Palmer, D. E. Fishbaugher, J. P. Schmitt, Y. Wang, N. R. Alpert, C. E. Seidman, J. G. Seidman, P. VanBuren, and D. W. Maughan
Differential cross-bridge kinetics of FHC myosin mutations R403Q and R453C in heterozygous mouse myocardium
Am J Physiol Heart Circ Physiol, July 1, 2004; 287(1): H91 - H99.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. B. Foster, T. Noguchi, P. VanBuren, A. M. Murphy, and J. E. Van Eyk
C-Terminal Truncation of Cardiac Troponin I Causes Divergent Effects on ATPase and Force: Implications for the Pathophysiology of Myocardial Stunning
Circ. Res., November 14, 2003; 93(10): 917 - 924.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Siedner, M. Kruger, M. Schroeter, D. Metzler, W. Roell, B. K Fleischmann, J. Hescheler, G. Pfitzer, and R. Stehle
Developmental changes in contractility and sarcomeric proteins from the early embryonic to the adult stage in the mouse heart
J. Physiol., April 15, 2003; 548(2): 493 - 505.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. J. Biesiadecki, B. D. Elder, Z.-B. Yu, and J.-P. Jin
Cardiac Troponin T Variants Produced by Aberrant Splicing of Multiple Exons in Animals with High Instances of Dilated Cardiomyopathy
J. Biol. Chem., December 20, 2002; 277(52): 50275 - 50285.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. V. Gomes, G. Guzman, J. Zhao, and J. D. Potter
Cardiac Troponin T Isoforms Affect the Ca2+ Sensitivity and Inhibition of Force Development. INSIGHTS INTO THE ROLE OF TROPONIN T ISOFORMS IN THE HEART
J. Biol. Chem., September 13, 2002; 277(38): 35341 - 35349.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.