AJP - Heart pressure measurements
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (January 3, 2002). doi:10.1152/ajpheart.00984.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/5/H1635    most recent
00984.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diep, Q. N
Right arrow Articles by Schiffrin, E. L
Right arrow Search for Related Content
PubMed
Right arrow Articles by Diep, Q. N
Right arrow Articles by Schiffrin, E. L

Articles in PresS, published online ahead of print January 3, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.00984.2001
Submitted on November 12, 2001
Accepted on January 2, 2002

Effect of AT1 Receptor Blockade on Cardiac Apoptosis in Angiotensin II-induced Hypertension

Quy N Diep1, Mohammed El Mabrouk1, Ping Yue1, and Ernesto L Schiffrin1*

1 Laboratory of Experimental Hypertension, Clinical Research Institute of Montreal, Montreal, Quebec, Canada

* To whom correspondence should be addressed. E-mail: diepq{at}ircm.qc.ca.

Angiotensin II (Ang II) via AT1 receptors induces apoptosis in cardiomyocytes in vitro. We tested the hypothesis that in vivo AT1 receptor stimulation is accompanied by cardiac apoptosis, and attempted to elucidate molecular mechanisms involved in the death-signaling pathway. Male Sprague Dawley rats received Ang II (120 ng/kg/min sc.) for 7 days with or without the AT1 receptor antagonist losartan (10 mg/kg/day orally). Cardiac function was assessed by echocardiography. Apoptosis in the heart was detected and quantified by in situ terminal deoxynucleotide transferase dUTP-nick-end-labeling (TUNEL) and radiolabeled DNA laddering. Expression of bax, bcl-2, caspase 3, AT1 and AT2 receptors were examined by Western blot analysis. Activity of caspase 3 was also measured by a fluorometric immunosorbent enzyme assay. Tail cuff systolic blood pressure (mmHg) was elevated (p<0.01, n=6) in Ang II-infused rats (173±3) vs. controls (111±2) and reduced by losartan (134±4). Cardiac function was essentially unchanged in Ang II-infused rats. Increased internucleosomal DNA cleavage by TUNEL assay and radiolabeled DNA laddering showed results compatible with enhanced cardiomyocyte apoptosis in the heart of Ang-II infused rats. The bax/bcl-2 ratio, expression of the active form of caspase 3 (17kDa) and activity of caspase 3 in the heart of Ang II-group increased more than 2-fold above control. Protein expression of AT1 and AT2 receptors was significantly increased in Ang II-infused rats compared to control rats. Losartan-treated Ang II-infused rats exhibited normalized apoptosis, bax, caspase 3 activity, and AT1 receptors. Ang II stimulation of AT1 receptors in the heart in vivo is associated with increased rate of apoptosis without major hemodynamic consequences. Bax and caspase 3 are involved in the apoptotic signaling pathway in this experimental paradigm.




This article has been cited by other articles:


Home page
 HypertensionHome page
A.-i. Shigenaga, K. Tamura, H. Wakui, S.-i. Masuda, K. Azuma, Y. Tsurumi-Ikeya, M. Ozawa, M. Mogi, M. Matsuda, K. Uchino, et al.
Effect of Olmesartan on Tissue Expression Balance Between Angiotensin II Receptor and Its Inhibitory Binding Molecule
Hypertension, October 1, 2008; 52(4): 672 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S.-D. Lee, C.-H. Chu, E.-J. Huang, M.-C. Lu, J.-Y. Liu, C.-J. Liu, H.-H. Hsu, J. A. Lin, W.-W. Kuo, and C.-Y. Huang
Roles of insulin-like growth factor II in cardiomyoblast apoptosis and in hypertensive rat heart with abdominal aorta ligation.
Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E306 - E314.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Paul, A. Poyan Mehr, and R. Kreutz
Physiology of local Renin-Angiotensin systems.
Physiol Rev, July 1, 2006; 86(3): 747 - 803.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Tian, J. Liu, P. Bitterman, and R. J. Bache
Angiotensin II modulates nitric oxide-induced cardiac fibroblast apoptosis by activation of AKT/PKB
Am J Physiol Heart Circ Physiol, August 7, 2003; 285(3): H1105 - H1112.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. A. Fortuno, A. Gonzalez, S. Ravassa, B. Lopez, and J. Diez
Clinical implications of apoptosis in hypertensive heart disease
Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1495 - H1506.
[Full Text] [PDF]

Home page
 Eur Heart J SupplHome page
J. Soler-Soler and D. Garcia-Dorado
How to best to counteract the enemies? By blocking neurohormonal activation
Eur. Heart J. Suppl., November 1, 2002; 4(suppl_G): G45 - G50.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.