AJP - Heart AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (February 13, 2003). doi:10.1152/ajpheart.00999.2002
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Videos
Right arrow All Versions of this Article:
284/6/H2213    most recent
00999.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grayson, M. H.
Right arrow Articles by Chaplin, D. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grayson, M. H.
Right arrow Articles by Chaplin, D. D.
Submitted on November 18, 2002
Accepted on February 4, 2003

Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node

Mitchell H. Grayson1*, Richard S. Hotchkiss2, Irene E. Karl1, Michael J. Holtzman3, and David D. Chaplin4

1 Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA
2 Department of Cell Biology, Washington University School of Medicine, Saint Louis, MO, USA; Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA
3 Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, Saint Louis, MO, USA
4 Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA

* To whom correspondence should be addressed. E-mail: wheeze{at}allergist.com.

Lymphocyte rolling velocity is determined largely by interactions between leukocyte {alpha}4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. Utilizing real-time intravital confocal microscopy, we find rolling velocities of T lymphocytes in murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in murine spleen, rolling velocities of T lymphocytes are not influenced by loss of L-selectin and CD49d. Using FITC-dextran and TIE2-GFP mice, we further define the microvascular compartments of the spleen, and show that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs, and suggest the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.




This article has been cited by other articles:


Home page
 BloodHome page
S. Yakubenia, D. Frommhold, D. Scholch, C. C. Hellbusch, C. Korner, B. Petri, C. Jones, U. Ipe, M. G. Bixel, R. Krempien, et al.
Leukocyte trafficking in a mouse model for leukocyte adhesion deficiency II/congenital disorder of glycosylation IIc
Blood, August 15, 2008; 112(4): 1472 - 1481.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
M. H. Grayson, D. Cheung, M. M. Rohlfing, R. Kitchens, D. E. Spiegel, J. Tucker, J. T. Battaile, Y. Alevy, L. Yan, E. Agapov, et al.
Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia
J. Exp. Med., October 29, 2007; 204(11): 2759 - 2769.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.