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Am J Physiol Heart Circ Physiol (February 13, 2003). doi:10.1152/ajpheart.00999.2002
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Submitted on November 18, 2002
Accepted on February 4, 2003

Intravital microscopy comparing T lymphocyte trafficking to the spleen and the mesenteric lymph node

Mitchell H. Grayson1*, Richard S. Hotchkiss2, Irene E. Karl1, Michael J. Holtzman3, and David D. Chaplin4

1 Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA
2 Department of Cell Biology, Washington University School of Medicine, Saint Louis, MO, USA; Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA
3 Department of Internal Medicine, Washington University School of Medicine, Saint Louis, MO, USA; Department of Anesthesiology, Washington University School of Medicine, Saint Louis, MO, USA
4 Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA

* To whom correspondence should be addressed. E-mail: wheeze{at}allergist.com.

Lymphocyte rolling velocity is determined largely by interactions between leukocyte {alpha}4-integrin (CD49d) and L-selectin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in mesenteric postcapillary venules and Peyer's patch high endothelial venules (HEVs). The role of these interactions in other tissue sites of lymphocyte emigration is not known. Utilizing real-time intravital confocal microscopy, we find rolling velocities of T lymphocytes in murine mesenteric lymph node (MLN) HEV also depend on L-selectin and CD49d. However, in murine spleen, rolling velocities of T lymphocytes are not influenced by loss of L-selectin and CD49d. Using FITC-dextran and TIE2-GFP mice, we further define the microvascular compartments of the spleen, and show that adherence of T cells is localized to regions in the white pulp that are not lined by endothelial cells and have shear rates similar to bone marrow sinusoids. These results establish that T cell trafficking to the spleen differs from trafficking to other secondary lymphoid organs, and suggest the mechanical properties of the blood-filtering role of the spleen are important in T cell accumulation in the organ.




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