AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Am J Physiol Heart Circ Physiol (January 8, 2004). doi:10.1152/ajpheart.01000.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/5/H1895    most recent
01000.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cooper, D.
Right arrow Articles by Granger, D N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cooper, D.
Right arrow Articles by Granger, D N.
Submitted on October 23, 2003
Accepted on January 5, 2004

Leukocyte-dependency of platelet adhesion in postcapillary venules

Dianne Cooper1, Janice Russell1, Keith D. Chitman1, Matthew C. Williams1, Robert E. Wolf2, and D Neil Granger1*

1 Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, LA, USA
2 Center of Excellence in Arthritis Rheumatology, Louisiana State University Health Sciences Center, Shreveport, LA, USA

* To whom correspondence should be addressed. E-mail: dgrang{at}lsuhsc.edu.

Reperfusion of ischemic tissues results in development of a pro-inflammatory, prothrombogenic phenotype, culminating in the recruitment of leukocytes and platelets within postcapillary venules. Recent studies have indicated an inter-dependency of platelet and leukocyte adhesion, suggesting that heterotypic blood cell interactions may account for post-ischemic platelet recruitment. The objectives of this study were to: 1) determine whether ischemia/reperfusion (I/R)-induced platelet recruitment is leukocyte-dependent and 2) quantify the contributions of leukocytes and endothelial cells in this platelet recruitment. Intravital microscopy was used to monitor the recruitment of fluorescently-labeled platelets in post-capillary venules of the small intestine after 45min ischemia and 4h reperfusion. To assess the leukocyte-dependency of platelet adhesion, platelets from wild-type mice were infused into mice deficient in neutrophils and/or lymphocytes and mice deficient in key leukocyte adhesion molecules (CD18 and ICAM-1). These anti-leukocyte strategies resulted in significantly reduced platelet recruitment. Simultaneous visualization of platelets and leukocytes enabled quantification of leukocyte-dependent and endothelium-dependent platelet adhesion. It was observed, that in wild-type animals, 74% of I/R-induced platelet adhesion was a result of plateletleukocyte interactions. While the majority of adherent platelets were associated with leukocytes, less than 50% of adherent leukocytes were platelet-bearing, suggesting not all adherent leukocytes support platelet adhesion. These results are consistent with leukocytes playing a major role in supporting I/Rinduced platelet adhesion.







HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.