Reperfusion of ischemic tissues results in development of a pro-inflammatory, prothrombogenic phenotype, culminating in the recruitment of leukocytes and platelets within postcapillary venules. Recent studies have indicated an inter-dependency of platelet and leukocyte adhesion, suggesting that heterotypic blood cell interactions may account for post-ischemic platelet recruitment. The objectives of this study were to: 1) determine whether ischemia/reperfusion (I/R)-induced platelet recruitment is leukocyte-dependent and 2) quantify the contributions of leukocytes and endothelial cells in this platelet recruitment. Intravital microscopy was used to monitor the recruitment of fluorescently-labeled platelets in post-capillary venules of the small intestine after 45min ischemia and 4h reperfusion. To assess the leukocyte-dependency of platelet adhesion, platelets from wild-type mice were infused into mice deficient in neutrophils and/or lymphocytes and mice deficient in key leukocyte adhesion molecules (CD18 and ICAM-1). These anti-leukocyte strategies resulted in significantly reduced platelet recruitment. Simultaneous visualization of platelets and leukocytes enabled quantification of leukocyte-dependent and endothelium-dependent platelet adhesion. It was observed, that in wild-type animals, 74% of I/R-induced platelet adhesion was a result of plateletleukocyte interactions. While the majority of adherent platelets were associated with leukocytes, less than 50% of adherent leukocytes were platelet-bearing, suggesting not all adherent leukocytes support platelet adhesion. These results are consistent with leukocytes playing a major role in supporting I/Rinduced platelet adhesion.