| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Pharmacology, The University of Tokushima School of Medicine, Tokushima, Tokushima, Japan
2 Department of Integrative Physiology, The University of Tokushima School of Medicine, Tokushima, Tokushima, Japan
* To whom correspondence should be addressed. E-mail: tamaki{at}basic.med.tokushima-u.ac.jp.
The measurement of HbNO in whole blood by the EPR method seems relevant for the assessment of systemic NO levels. However, ceruloplasmin and unknown radical species overlap the same magnetic field to that of HbNO. Then, to reveal the EPR spectrum of HbNO, we introduced the EPR signal subtraction method, which is based on the computer assisted subtraction of the digitized EPR spectrum of HbNO-depleted blood from that of sample blood using the software.
Rats were treated with L-NAME (120 mg/kg/day) for a week to obtain HbNO-depleted blood. When this method was applied to the analysis of untreated fresh whole blood, the 5-coordinate state of HbNO was observed. HbNO concentration in pentobarbital-anesthetized rats, was augmented (
[HbNO] =1.6 - 5.5 µM) by infusion of L-arginine (0.2 - 0.6 g/kg) but not D-arginine.
Using this method, we attempted to evaluate the effects of temocapril on HbNO dynamics in a L-NAME-induced rat endothelial dysfunction model. The oral administration of L-NAME for 2 weeks induced a serious hypertension, and HbNO concentration was reduced ([HbNO] = 5.7 µM ). Co-administration of temocapril dose-dependently improved both changes in blood pressure and the systemic HbNO concentration.
In this study, we succeeded in measuring the blood HbNO level as an index of NO by the EPR HbNO signal subtraction method. We also demonstrated that temocapril improves abnormalities of NO dynamics in the L-NAME-induced endothelial dysfunction rats using the EPR HbNO signal subtraction method.
This article has been cited by other articles:
|
|
 |
|
  Y. Kanematsu, K. Yamaguchi, H. Ohnishi, Y. Motobayashi, K. Ishizawa, Y. Izawa, K. Kawazoe, S. Kondo, S. Kagami, S. Tomita, et al. Dietary doses of nitrite restore circulating nitric oxide level and improve renal injury in L-NAME-induced hypertensive rats Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1457 - F1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Derakhshan, G. Hao, and S. S. Gross Balancing reactivity against selectivity: The evolution of protein S-nitrosylation as an effector of cell signaling by nitric oxide Cardiovasc Res, July 15, 2007; 75(2): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsuchiya, Y. Kanematsu, M. Yoshizumi, H. Ohnishi, K. Kirima, Y. Izawa, M. Shikishima, T. Ishida, S. Kondo, S. Kagami, et al. Nitrite is an alternative source of NO in vivo Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2163 - H2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Okamoto, K. Tsuchiya, Y. Kanematsu, Y. Izawa, M. Yoshizumi, S. Kagawa, and T. Tamaki Nitrite-derived nitric oxide formation following ischemia-reperfusion injury in kidney Am J Physiol Renal Physiol, January 1, 2005; 288(1): F182 - F187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Foster, J. R. Pawloski, D. J. Singel, and J. S. Stamler Role of Circulating S-Nitrosothiols in Control of Blood Pressure Hypertension, January 1, 2005; 45(1): 15 - 17. [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Stamler S-Nitrosothiols in the Blood: Roles, Amounts, and Methods of Analysis Circ. Res., March 5, 2004; 94(4): 414 - 417. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
