| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Pharmaceutical Chemistry, University of Kansas, Lawrence, Kansas, USA
2 Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
* To whom correspondence should be addressed. E-mail: schoneic{at}ukans.edu.
Proteomic techniques were used to identify cardiac proteins from whole heart homogenate and heart mitochondria of Fisher 344/Brown Norway (BN) F1 rats, which suffer protein nitration as a consequence of biological aging. Soluble Proteins from young (5 months) and old (26 months) animals were separated by 1-D and 2-D gel electrophoresis (1DE and 2DE, respectively). 1-D and 2-D Western blots with an anti-nitrotyrosine antibody show an age-related increase in the immunoresponse of a few specific proteins, which were identified by nano-electrospray-ionization MS/MS (NSI-MS/MS). Complementary, proteins were immunoprecipitated with an immobilized anti-nitrotyrosine antibody, followed by NSI-MS/MS analysis. A total of 48 proteins were putatively identified. Among the identified proteins are 
-enolase, -aldolase, desmin, aconitate hydratase, methylmalonate semialdehyde dehydrogenase, 3-ketoacyl-CoA thiolase, Acetyl CoA-Acetyltransferase, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), malate dehydrogenase, creatine kinase, electron transfer flavoprotein, manganese superoxide dismutase (MnSOD), F1-Atpase and the voltage dependent anion channel. Some contaminating blood proteins, transferrin and fibrinogen 
-chain precusor, show increased levels of nitration as well. MS/MS analysis located nitration at Y105 of the electron transfer flavoprotein. Among the identified proteins, there are important enzymes responsible for energy production, metabolism as well as proteins involved in the structural integrity of the cells. Our results are consistent with age-dependent increased oxidative stress and with free radical-dependent damage of proteins. Possibly the oxidative modifications of the identified proteins contribute to the age-dependent degeneration and functional decline of the heart proteins.
This article has been cited by other articles:
|
|
 |
|
  A. Jahangir, S. Sagar, and A. Terzic Aging and cardioprotection J Appl Physiol, December 1, 2007; 103(6): 2120 - 2128. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ferdinandy, R. Schulz, and G. F. Baxter Interaction of Cardiovascular Risk Factors with Myocardial Ischemia/Reperfusion Injury, Preconditioning, and Postconditioning Pharmacol. Rev., December 1, 2007; 59(4): 418 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Judge and C. Leeuwenburgh Cardiac mitochondrial bioenergetics, oxidative stress, and aging Am J Physiol Cell Physiol, June 1, 2007; 292(6): C1983 - C1992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Navarro and A. Boveris The mitochondrial energy transduction system and the aging process Am J Physiol Cell Physiol, February 1, 2007; 292(2): C670 - C686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pacher, J. S. Beckman, and L. Liaudet Nitric Oxide and Peroxynitrite in Health and Disease Physiol Rev, January 1, 2007; 87(1): 315 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Thompson, D. Durand, N. A. Fugere, and D. A. Ferrington Myosin and actin expression and oxidation in aging muscle J Appl Physiol, December 1, 2006; 101(6): 1581 - 1587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Fugere, D. A. Ferrington, and L. V. Thompson Protein nitration with aging in the rat semimembranosus and soleus muscles. J. Gerontol. A Biol. Sci. Med. Sci., August 1, 2006; 61(8): 806 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xu, J. Ying, B. Jiang, W. Guo, T. Adachi, V. Sharov, H. Lazar, J. Menzoian, T. V. Knyushko, D. Bigelow, et al. Detection of sequence-specific tyrosine nitration of manganese SOD and SERCA in cardiovascular disease and aging Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2220 - H2227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Viappiani and R. Schulz Detection of specific nitrotyrosine-modified proteins as a marker of oxidative stress in cardiovascular disease Am J Physiol Heart Circ Physiol, June 1, 2006; 290(6): H2167 - H2168. [Full Text] [PDF]
|
|
|
|
 |
|
 A. Borbely, A. Toth, I. Edes, L. Virag, J. G. Papp, A. Varro, W. J. Paulus, J. van der Velden, G. J.M. Stienen, and Z. Papp Peroxynitrite-induced {alpha}-actinin nitration and contractile alterations in isolated human myocardial cells Cardiovasc Res, August 1, 2005; 67(2): 225 - 233. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
