| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan
* To whom correspondence should be addressed. E-mail: nagayann{at}hsp.ncvc.go.jp.
Mesenchymal stem cells (MSCs) are pluripotent cells which differentiate into a variety of cells including cardiomyocytes and endothelial cells. However, little information is available regarding the therapeutic potency of systemically delivered MSCs for myocardial infarction. Accordingly, we investigated whether intravenously transplanted MSCs induce angiogenesis and myogenesis and improve cardiac function in rats with acute myocardial infarction. MSCs were isolated from bone marrow aspirates of isogenic adult rats and expanded ex vivo. Three hours after coronary ligation, 5 x 106 MSCs (MSC group, n = 12) or vehicle (Control group, n = 12) was intravenously administered to Lewis rats. Transplanted MSCs were preferentially attracted to the infarcted myocardium but not to the noninfarcted myocardium. The engrafted MSCs were positive for cardiac markers: desmin, cardiac troponin T, and connexin-43. On the other hand, some of the transplanted MSCs were positive for von Willebrand factor and formed vascular structures. Capillary density was markedly increased after MSC transplantation. Cardiac infarct size in the MSC group was significantly smaller than that in the Control group (24 ± 2 versus 33 ± 2%, p < 0.05). MSC transplantation decreased left ventricular end-diastolic pressure and increased left ventricular maximum dP/dt (both p < 0.05 vs. Control group). These results suggest that intravenous administration of MSCs improves cardiac function after acute myocardial infarction through enhancement of angiogenesis and myogenesis in the ischemic myocardium.
This article has been cited by other articles:
|
|
 |
|
  A. Shabbir, D. Zisa, G. Suzuki, and T. Lee Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: a noninvasive therapeutic regimen Am J Physiol Heart Circ Physiol, June 1, 2009; 296(6): H1888 - H1897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Otsu, S. Das, S. D. Houser, S. K. Quadri, S. Bhattacharya, and J. Bhattacharya Concentration-dependent inhibition of angiogenesis by mesenchymal stem cells Blood, April 30, 2009; 113(18): 4197 - 4205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-C. Lee, H.-C. Hsu, W.-Y. I. Tseng, C.-Y. Chen, H.-J. Lin, Y.-L. Ho, M.-J. Su, and M.-F. Chen Cell therapy generates a favourable chemokine gradient for stem cell recruitment into the infarcted heart in rabbits Eur J Heart Fail, March 1, 2009; 11(3): 238 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Vela, G. V. Silva, J. A.R. Assad, A. L.S. Sousa, S. Coulter, M. R. Fernandes, E. C. Perin, J. T. Willerson, and L. M. Buja Histopathological Study of Healing After Allogenic Mesenchymal Stem Cell Delivery in Myocardial Infarction in Dogs J. Histochem. Cytochem., February 1, 2009; 57(2): 167 - 176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gnecchi, Z. Zhang, A. Ni, and V. J. Dzau Paracrine Mechanisms in Adult Stem Cell Signaling and Therapy Circ. Res., November 21, 2008; 103(11): 1204 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Yao, R Huang, J Qian, J Cui, L Ge, Y Li, F Zhang, H Shi, D Huang, S Zhang, et al. Administration of intracoronary bone marrow mononuclear cells on chronic myocardial infarction improves diastolic function Heart, September 1, 2008; 94(9): 1147 - 1153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Carr, D. J. Stuckey, L. Tatton, D. J. Tyler, S. J. M. Hale, D. Sweeney, J. E. Schneider, E. Martin-Rendon, G. K. Radda, S. E. Harding, et al. Bone marrow-derived stromal cells home to and remain in the infarcted rat heart but fail to improve function: an in vivo cine-MRI study Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H533 - H542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Au, J. Tam, D. Fukumura, and R. K. Jain Bone marrow-derived mesenchymal stem cells facilitate engineering of long-lasting functional vasculature Blood, May 1, 2008; 111(9): 4551 - 4558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shi, J. Li, L. Liao, B. Chen, B. Li, L. Chen, H. Jia, and R. C. Zhao Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice Haematologica, July 1, 2007; 92(7): 897 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Togel, K. Weiss, Y. Yang, Z. Hu, P. Zhang, and C. Westenfelder Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1626 - F1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. De Becker, P. Van Hummelen, M. Bakkus, I. Vande Broek, J. De Wever, M. De Waele, and I. Van Riet Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3 Haematologica, April 1, 2007; 92(4): 440 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Tran, P. R. Franken, F. Maskali, J. Nloga, P. Maureira, S. Poussier, F. Groubatch, C. Vanhove, J.-P. Villemot, and P.-Y. Marie Intramyocardial Implantation of Bone Marrow-Derived Stem Cells Enhances Perfusion in Chronic Myocardial Infarction: Dependency on Initial Perfusion Depth and Follow-up Assessed by Gated Pinhole SPECT J. Nucl. Med., March 1, 2007; 48(3): 405 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pevsner-Fischer, V. Morad, M. Cohen-Sfady, L. Rousso-Noori, A. Zanin-Zhorov, S. Cohen, I. R. Cohen, and D. Zipori Toll-like receptors and their ligands control mesenchymal stem cell functions Blood, February 15, 2007; 109(4): 1422 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Pallante, I. Duignan, D. Okin, A. Chin, M. C. Bressan, T. Mikawa, and J. M. Edelberg Bone Marrow Oct3/4+ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms Circ. Res., January 5, 2007; 100(1): e1 - e11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Amado, K. H. Schuleri, A. P. Saliaris, A. J. Boyle, R. Helm, B. Oskouei, M. Centola, V. Eneboe, R. Young, J. A.C. Lima, et al. Multimodality Noninvasive Imaging Demonstrates In Vivo Cardiac Regeneration After Mesenchymal Stem Cell Therapy J. Am. Coll. Cardiol., November 21, 2006; 48(10): 2116 - 2124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Rosen Are Stem Cells Drugs?: The Regulation of Stem Cell Research and Development Circulation, October 31, 2006; 114(18): 1992 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Kunter, S. Rong, Z. Djuric, P. Boor, G. Muller-Newen, D. Yu, and J. Floege Transplanted Mesenchymal Stem Cells Accelerate Glomerular Healing in Experimental Glomerulonephritis J. Am. Soc. Nephrol., August 1, 2006; 17(8): 2202 - 2212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kanki-Horimoto, H. Horimoto, S. Mieno, K. Kishida, F. Watanabe, E. Furuya, and T. Katsumata Implantation of Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase Improves Right Ventricular Impairments Caused by Pulmonary Hypertension Circulation, July 4, 2006; 114(1_suppl): I-181 - I-185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kanki-Horimoto, H. Horimoto, S. Mieno, K. Kishida, F. Watanabe, E. Furuya, and T. Katsumata Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase Circulation, July 4, 2006; 114(1_suppl): I-327 - I-330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y Wang, H E Johnsen, S Mortensen, L Bindslev, R Sejersten Ripa, M Haack-Sorensen, E Jorgensen, W Fang, and J Kastrup Changes in circulating mesenchymal stem cells, stem cell homing factor, and vascular growth factors in patients with acute ST elevation myocardial infarction treated with primary percutaneous coronary intervention Heart, June 1, 2006; 92(6): 768 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Y. Khakoo, S. Pati, S. A. Anderson, W. Reid, M. F. Elshal, I. I. Rovira, A. T. Nguyen, D. Malide, C. A. Combs, G. Hall, et al. Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma J. Exp. Med., May 15, 2006; 203(5): 1235 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schaefer, G. P. Meyer, M. Fuchs, G. Klein, M. Kaplan, K. C. Wollert, and H. Drexler Impact of intracoronary bone marrow cell transfer on diastolic function in patients after acute myocardial infarction: results from the BOOST trial Eur. Heart J., April 2, 2006; 27(8): 929 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Furuta, S. Miyoshi, Y. Itabashi, T. Shimizu, S. Kira, K. Hayakawa, N. Nishiyama, K. Tanimoto, Y. Hagiwara, T. Satoh, et al. Pulsatile Cardiac Tissue Grafts Using a Novel Three-Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, In Vivo Circ. Res., March 17, 2006; 98(5): 705 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Minguell and A. Erices Mesenchymal Stem Cells and the Treatment of Cardiac Disease Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Togel, Z. Hu, K. Weiss, J. Isaac, C. Lange, and C. Westenfelder Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms Am J Physiol Renal Physiol, July 1, 2005; 289(1): F31 - F42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Haider and M. Ashraf Bone marrow stem cell transplantation for cardiac repair Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2557 - H2567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hanabusa, N. Nagaya, T. Iwase, T. Itoh, S. Murakami, Y. Shimizu, W. Taki, K. Miyatake, and K. Kangawa Adrenomedullin Enhances Therapeutic Potency of Mesenchymal Stem Cells After Experimental Stroke in Rats Stroke, April 1, 2005; 36(4): 853 - 858. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
