AJP - Heart Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol (July 29, 2004). doi:10.1152/ajpheart.01071.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/6/H2670    most recent
01071.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagaya, N.
Right arrow Articles by Kitamura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagaya, N.
Right arrow Articles by Kitamura, S.
Submitted on November 10, 2003
Accepted on July 13, 2004

Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis

Noritoshi Nagaya1*, Takafumi Fujii1, Takashi Iwase1, Hajime Ohgushi1, Takefumi Itoh1, Masaaki Uematsu1, Masakazu Yamagishi1, Hidezo Mori1, Kenji Kangawa1, and Soichiro Kitamura1

1 Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan

* To whom correspondence should be addressed. E-mail: nagayann{at}hsp.ncvc.go.jp.

Mesenchymal stem cells (MSCs) are pluripotent cells which differentiate into a variety of cells including cardiomyocytes and endothelial cells. However, little information is available regarding the therapeutic potency of systemically delivered MSCs for myocardial infarction. Accordingly, we investigated whether intravenously transplanted MSCs induce angiogenesis and myogenesis and improve cardiac function in rats with acute myocardial infarction. MSCs were isolated from bone marrow aspirates of isogenic adult rats and expanded ex vivo. Three hours after coronary ligation, 5 x 106 MSCs (MSC group, n = 12) or vehicle (Control group, n = 12) was intravenously administered to Lewis rats. Transplanted MSCs were preferentially attracted to the infarcted myocardium but not to the noninfarcted myocardium. The engrafted MSCs were positive for cardiac markers: desmin, cardiac troponin T, and connexin-43. On the other hand, some of the transplanted MSCs were positive for von Willebrand factor and formed vascular structures. Capillary density was markedly increased after MSC transplantation. Cardiac infarct size in the MSC group was significantly smaller than that in the Control group (24 ± 2 versus 33 ± 2%, p < 0.05). MSC transplantation decreased left ventricular end-diastolic pressure and increased left ventricular maximum dP/dt (both p < 0.05 vs. Control group). These results suggest that intravenous administration of MSCs improves cardiac function after acute myocardial infarction through enhancement of angiogenesis and myogenesis in the ischemic myocardium.




This article has been cited by other articles:


Home page
 Circ. Res.Home page
M. Gnecchi, Z. Zhang, A. Ni, and V. J. Dzau
Paracrine Mechanisms in Adult Stem Cell Signaling and Therapy
Circ. Res., November 21, 2008; 103(11): 1204 - 1219.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
K Yao, R Huang, J Qian, J Cui, L Ge, Y Li, F Zhang, H Shi, D Huang, S Zhang, et al.
Administration of intracoronary bone marrow mononuclear cells on chronic myocardial infarction improves diastolic function
Heart, September 1, 2008; 94(9): 1147 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
L. da Silva Meirelles, A. I. Caplan, and N. B. Nardi
In Search of the In Vivo Identity of Mesenchymal Stem Cells
Stem Cells, September 1, 2008; 26(9): 2287 - 2299.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Morigi, M. Introna, B. Imberti, D. Corna, M. Abbate, C. Rota, D. Rottoli, A. Benigni, N. Perico, C. Zoja, et al.
Human Bone Marrow Mesenchymal Stem Cells Accelerate Recovery of Acute Renal Injury and Prolong Survival in Mice
Stem Cells, August 1, 2008; 26(8): 2075 - 2082.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. A. Carr, D. J. Stuckey, L. Tatton, D. J. Tyler, S. J. M. Hale, D. Sweeney, J. E. Schneider, E. Martin-Rendon, G. K. Radda, S. E. Harding, et al.
Bone marrow-derived stromal cells home to and remain in the infarcted rat heart but fail to improve function: an in vivo cine-MRI study
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H533 - H542.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Au, J. Tam, D. Fukumura, and R. K. Jain
Bone marrow-derived mesenchymal stem cells facilitate engineering of long-lasting functional vasculature
Blood, May 1, 2008; 111(9): 4551 - 4558.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G. Chamberlain, J. Fox, B. Ashton, and J. Middleton
Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing
Stem Cells, November 1, 2007; 25(11): 2739 - 2749.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S.-C. Hung, R. R. Pochampally, S.-C. Chen, S.-C. Hsu, and D. J. Prockop
Angiogenic Effects of Human Multipotent Stromal Cell Conditioned Medium Activate the PI3K-Akt Pathway in Hypoxic Endothelial Cells to Inhibit Apoptosis, Increase Survival, and Stimulate Angiogenesis
Stem Cells, September 1, 2007; 25(9): 2363 - 2370.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
W. Li, N. Ma, L.-L. Ong, C. Nesselmann, C. Klopsch, Y. Ladilov, D. Furlani, C. Piechaczek, J. M. Moebius, K. Lutzow, et al.
Bcl-2 Engineered MSCs Inhibited Apoptosis and Improved Heart Function
Stem Cells, August 1, 2007; 25(8): 2118 - 2127.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
M. Shi, J. Li, L. Liao, B. Chen, B. Li, L. Chen, H. Jia, and R. C. Zhao
Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice
Haematologica, July 1, 2007; 92(7): 897 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
I. A. Potapova, G. R. Gaudette, P. R. Brink, R. B. Robinson, M. R. Rosen, I. S. Cohen, and S. V. Doronin
Mesenchymal Stem Cells Support Migration, Extracellular Matrix Invasion, Proliferation, and Survival of Endothelial Cells In Vitro
Stem Cells, July 1, 2007; 25(7): 1761 - 1768.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
P. A. Schweizer, U. Krause, R. Becker, A. Seckinger, A. Bauer, C. Hardt, V. Eckstein, A. D. Ho, M. Koenen, H. A. Katus, et al.
Atrial-Radiofrequency Catheter Ablation Mediated Targeting of Mesenchymal Stromal Cells
Stem Cells, June 1, 2007; 25(6): 1546 - 1551.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Togel, K. Weiss, Y. Yang, Z. Hu, P. Zhang, and C. Westenfelder
Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1626 - F1635.
[Abstract] [Full Text] [PDF]

Home page
 haematolHome page
A. De Becker, P. Van Hummelen, M. Bakkus, I. Vande Broek, J. De Wever, M. De Waele, and I. Van Riet
Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3
Haematologica, April 1, 2007; 92(4): 440 - 449.
[Abstract] [Full Text] [PDF]

Home page
 JNMHome page
N. Tran, P. R. Franken, F. Maskali, J. Nloga, P. Maureira, S. Poussier, F. Groubatch, C. Vanhove, J.-P. Villemot, and P.-Y. Marie
Intramyocardial Implantation of Bone Marrow-Derived Stem Cells Enhances Perfusion in Chronic Myocardial Infarction: Dependency on Initial Perfusion Depth and Follow-up Assessed by Gated Pinhole SPECT
J. Nucl. Med., March 1, 2007; 48(3): 405 - 412.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Pevsner-Fischer, V. Morad, M. Cohen-Sfady, L. Rousso-Noori, A. Zanin-Zhorov, S. Cohen, I. R. Cohen, and D. Zipori
Toll-like receptors and their ligands control mesenchymal stem cell functions
Blood, February 15, 2007; 109(4): 1422 - 1432.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. A. Pallante, I. Duignan, D. Okin, A. Chin, M. C. Bressan, T. Mikawa, and J. M. Edelberg
Bone Marrow Oct3/4+ Cells Differentiate Into Cardiac Myocytes via Age-Dependent Paracrine Mechanisms
Circ. Res., January 5, 2007; 100(1): e1 - e11.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. Schenk, N. Mal, A. Finan, M. Zhang, M. Kiedrowski, Z. Popovic, P. M. McCarthy, and M. S. Penn
Monocyte Chemotactic Protein-3 Is a Myocardial Mesenchymal Stem Cell Homing Factor
Stem Cells, January 1, 2007; 25(1): 245 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
L. C. Amado, K. H. Schuleri, A. P. Saliaris, A. J. Boyle, R. Helm, B. Oskouei, M. Centola, V. Eneboe, R. Young, J. A.C. Lima, et al.
Multimodality Noninvasive Imaging Demonstrates In Vivo Cardiac Regeneration After Mesenchymal Stem Cell Therapy
J. Am. Coll. Cardiol., November 21, 2006; 48(10): 2116 - 2124.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
M. R. Rosen
Are Stem Cells Drugs?: The Regulation of Stem Cell Research and Development
Circulation, October 31, 2006; 114(18): 1992 - 2000.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
U. Kunter, S. Rong, Z. Djuric, P. Boor, G. Muller-Newen, D. Yu, and J. Floege
Transplanted Mesenchymal Stem Cells Accelerate Glomerular Healing in Experimental Glomerulonephritis
J. Am. Soc. Nephrol., August 1, 2006; 17(8): 2202 - 2212.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
D. J. Stuckey, C. A. Carr, E. Martin-Rendon, D. J. Tyler, C. Willmott, P. J. Cassidy, S. J.M. Hale, J. E. Schneider, L. Tatton, S. E. Harding, et al.
Iron Particles for Noninvasive Monitoring of Bone Marrow Stromal Cell Engraftment into, and Isolation of Viable Engrafted Donor Cells from, the Heart
Stem Cells, August 1, 2006; 24(8): 1968 - 1975.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. Kanki-Horimoto, H. Horimoto, S. Mieno, K. Kishida, F. Watanabe, E. Furuya, and T. Katsumata
Implantation of Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase Improves Right Ventricular Impairments Caused by Pulmonary Hypertension
Circulation, July 4, 2006; 114(1_suppl): I-181 - I-185.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
S. Kanki-Horimoto, H. Horimoto, S. Mieno, K. Kishida, F. Watanabe, E. Furuya, and T. Katsumata
Synthetic Vascular Prosthesis Impregnated With Mesenchymal Stem Cells Overexpressing Endothelial Nitric Oxide Synthase
Circulation, July 4, 2006; 114(1_suppl): I-327 - I-330.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
Y Wang, H E Johnsen, S Mortensen, L Bindslev, R Sejersten Ripa, M Haack-Sorensen, E Jorgensen, W Fang, and J Kastrup
Changes in circulating mesenchymal stem cells, stem cell homing factor, and vascular growth factors in patients with acute ST elevation myocardial infarction treated with primary percutaneous coronary intervention
Heart, June 1, 2006; 92(6): 768 - 774.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
A. Y. Khakoo, S. Pati, S. A. Anderson, W. Reid, M. F. Elshal, I. I. Rovira, A. T. Nguyen, D. Malide, C. A. Combs, G. Hall, et al.
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma
J. Exp. Med., May 15, 2006; 203(5): 1235 - 1247.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
B.-R. Son, L. A. Marquez-Curtis, M. Kucia, M. Wysoczynski, A. R. Turner, J. Ratajczak, M. Z. Ratajczak, and A. Janowska-Wieczorek
Migration of Bone Marrow and Cord Blood Mesenchymal Stem Cells In Vitro Is Regulated by Stromal-Derived Factor-1-CXCR4 and Hepatocyte Growth Factor-c-met Axes and Involves Matrix Metalloproteinases
Stem Cells, May 1, 2006; 24(5): 1254 - 1264.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
A. Schaefer, G. P. Meyer, M. Fuchs, G. Klein, M. Kaplan, K. C. Wollert, and H. Drexler
Impact of intracoronary bone marrow cell transfer on diastolic function in patients after acute myocardial infarction: results from the BOOST trial
Eur. Heart J., April 2, 2006; 27(8): 929 - 935.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
A. Furuta, S. Miyoshi, Y. Itabashi, T. Shimizu, S. Kira, K. Hayakawa, N. Nishiyama, K. Tanimoto, Y. Hagiwara, T. Satoh, et al.
Pulsatile Cardiac Tissue Grafts Using a Novel Three-Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, In Vivo
Circ. Res., March 17, 2006; 98(5): 705 - 712.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
K. Tamama, V. H. Fan, L. G. Griffith, H. C. Blair, and A. Wells
Epidermal Growth Factor as a Candidate for Ex Vivo Expansion of Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells, March 1, 2006; 24(3): 686 - 695.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
J. J. Minguell and A. Erices
Mesenchymal Stem Cells and the Treatment of Cardiac Disease
Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Togel, Z. Hu, K. Weiss, J. Isaac, C. Lange, and C. Westenfelder
Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms
Am J Physiol Renal Physiol, July 1, 2005; 289(1): F31 - F42.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. K. Haider and M. Ashraf
Bone marrow stem cell transplantation for cardiac repair
Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2557 - H2567.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
K. Hanabusa, N. Nagaya, T. Iwase, T. Itoh, S. Murakami, Y. Shimizu, W. Taki, K. Miyatake, and K. Kangawa
Adrenomedullin Enhances Therapeutic Potency of Mesenchymal Stem Cells After Experimental Stroke in Rats
Stroke, April 1, 2005; 36(4): 853 - 858.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.