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Am J Physiol Heart Circ Physiol (July 11, 2002). doi:10.1152/ajpheart.01092.2001
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Articles in PresS, published online ahead of print July 11, 2002
Am J Physiol Heart Circ Physiol, 10.1152/ajpheart.01092.2001
Submitted on December 12, 2001
Accepted on July 3, 2002

Presence of a cellular renin angiotensin system in chromaffin cells of bovine adrenal medulla

Jun Ming Wang1, Dirk Slembrouck2, Junhui Tan3, Lut Arckens4, Frans H. H. Leenen3*, Pierre J. Courtoy5, and Werner P. De Potter2

1 Department of Medicine, University of Antwerp (UIA), Antwerp, Belgium; Hypertension Unit, University of Ottawa Heart Institute, Ottawa, Ontario, Canada
2 Department of Medicine, University of Antwerp (UIA), Antwerp, Belgium
3 Hypertension Unit, University of Ottawa Heart Institute, Ottawa, Ontario, Canada
4 Katholieke Universiteit Leuven, Zoological Institute, Leuven, Belgium
5 Cell Biology Unit, Louvain University Medical School, Brussels, Belgium

* To whom correspondence should be addressed. E-mail: fleenen{at}ottawaheart.ca.

The presence of a local renin-angiotensin system has been established in organs which serve as angiotensin targets. In this study, the expression of angiotensinogen mRNA, and subcellular localization of renin, angiotensin-converting enzyme and angiotensin II were investigated in bovine adrenal medullary cells in primary culture. By light microscopy, expression of angiotensinogen mRNA, immunoreactive renin, angiotensin-converting enzyme and angiotensin II were readily detectable only in the chromaffin cells. The density distribution of renin and angiotensin II in sucrose gradients suggested a concentration in chromaffin granules, a localization directly confirmed by immuno-electron microscopy. Reverse transcriptase-polymerase chain reaction and sequencing confirmed the expression of angiotensinogen in bovine chromaffin cells and adrenal medulla. In addition, in vitro autoradiography indicated that both angiotens in-converting enzyme and angiotensin type 1 receptors were present in the adrenal medulla. These results provide the first direct evidence that chromaffin cells in the adrenal medulla are not only the target for angiotensin, but should also be considered as potential local angiotensin-generating and -storing cells.




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