| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Cellular and Integrative Physiology, Indiana University Medical School, Indianapolis, Indiana, USA
* To whom correspondence should be addressed. E-mail: gbohlen{at}iupui.edu.
In cultured endothelial cells, 70-95% of extracellular L-arginine uptake has been attributed to the cationic amino acid transporter-1 protein (CAT-1). We tested the hypothesis that extracellular L-arginine entry into endothelial cells via CAT-1 plays a crucial role in endothelial nitric oxide (NO) production during in vivo conditions. Using L-lysine, the preferred amino acid transported by CAT-1, we competitively inhibited extracellular L-arginine transport into endothelial cells during conditions of NaCl hyperosmolarity, low oxygen, and flow increase. Our prior studies indicate each of these perturbations cause NO-dependent vasodilation. The perivascular NO concentration ([NO]) and blood flow were determined in the in vivo rat intestinal microvasculature. Suppression of extracellular L-arginine transport significantly and strongly inhibited increases in vascular [NO] and intestinal blood flow to NaCl hyperosmolarity, lowered oxygen tension, and increased flow. These results suggest that L-arginine from the extracellular space is accumulated by CAT-1. When CAT-1 mediated transport of extracellular L-arginine into endothelial cells was suppressed, the endothelial cell NO response to a wide range of physiological stimuli was strongly depressed.
This article has been cited by other articles:
|
|
 |
|
  X. Zhou, H. G. Bohlen, S. J. Miller, and J. L. Unthank NAD(P)H oxidase-derived peroxide mediates elevated basal and impaired flow-induced NO production in SHR mesenteric arteries in vivo Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H1008 - H1016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Bauser-Heaton, J. Song, and H. G. Bohlen Cerebral microvascular nNOS responds to lowered oxygen tension through a bumetanide-sensitive cotransporter and sodium-calcium exchanger Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2166 - H2173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Bauser-Heaton and H. G. Bohlen Cerebral microvascular dilation during hypotension and decreased oxygen tension: a role for nNOS Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2193 - H2201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Luiking and N. E. P. Deutz Biomarkers of Arginine and Lysine Excess J. Nutr., June 1, 2007; 137(6): 1662S - 1668S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Repetto, V. Pannunzio, F. Astort, C. Martinez Calejman, M. B. Moreno, O. P. Pignataro, and C. B. Cymeryng Characterization of L-arginine transport in adrenal cells: effect of ACTH Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E291 - E297. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
