| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Physiology, New York Medical College, Valhalla, New York, United States
2 Department of Physiology, New York Medical College, Valhalla, New York, United States
3 Biology, City College of New York, New York, New York, United States
4 Physiology, New York Medical College, Valhalla, United States
* To whom correspondence should be addressed. E-mail: zoltan_ungvari{at}nymc.edu.
The naked mole-rat (NMR; Heterocephalus glaber) is the longest-living rodent known (maximum lifespan potential [MLSP]: >28 years) and a unique model of successful aging showing attenuated declines in most physiological function. This study addresses age-related changes in endothelial function and production of reactive oxygen species in NMR arteries and vessels of shorter-living F344 rats (MLSP: ~3 years). Rats exhibit a significant age-dependent decline in acetylcholine-induced responses in carotid arteries over a two year age-range. In contrast, over a 10 year age range NO-mediated relaxation responses to acetylcholine and to the NO donor SNAP were unaltered in NMRs. Cellular O2.- and H2O2 production significantly increased with age in rat arteries, whereas they did not change substantially with age in NMR vessels. Indicators of apoptotic cell death (DNA fragmentation rate, caspase 3/7 activity) were significantly enhanced (~250-300%) in arteries of 2 year old rats. In contrast, vessels from 12 year old NMRs exhibited only a ~50 % increase in apoptotic cell death. In the hearts of NMRs (2 to 26 years old) expression of eNOS, antioxidant enzymes (Cu,Zn-SOD, Mn-SOD, catalase and glutathione peroxidase), the NAD(P)H oxidase subunit gp91phox and mitochondrial proteins (COX-IV, ATP synthase and porin, an indicator of mitochondrial mass) did not change significantly with age. Thus, long-living NMRs can maintain a youthful vascular function and cellular oxidant/antioxidant phenotype relatively longer and are better protected against aging-induced oxidative stress than shorter-living rats.
This article has been cited by other articles:
|
|
 |
|
  A. B. Salmon, S. Leonard, V. Masamsetti, A. Pierce, A. J. Podlutsky, N. Podlutskaya, A. Richardson, S. N. Austad, and A. R. Chaudhuri The long lifespan of two bat species is correlated with resistance to protein oxidation and enhanced protein homeostasis FASEB J, July 1, 2009; 23(7): 2317 - 2326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Zia, A. Csiszar, N. Labinskyy, F. Hu, G. Vinukonda, E. F. LaGamma, Z. Ungvari, and P. Ballabh Oxidative-Nitrosative Stress in a Rabbit Pup Model of Germinal Matrix Hemorrhage: Role of NAD(P)H Oxidase Stroke, June 1, 2009; 40(6): 2191 - 2198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Labinskyy, P. Mukhopadhyay, J. Toth, G. Szalai, M. Veres, G. Losonczy, J. T. Pinto, P. Pacher, P. Ballabh, A. Podlutsky, et al. Longevity is associated with increased vascular resistance to high glucose-induced oxidative stress and inflammatory gene expression in Peromyscus leucopus Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H946 - H956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Lesniewski, M. L. Connell, J. R. Durrant, B. J. Folian, M. C. Anderson, A. J. Donato, and D. R. Seals B6D2F1 Mice Are a Suitable Model of Oxidative Stress-Mediated Impaired Endothelium-Dependent Dilation With Aging J Gerontol A Biol Sci Med Sci, February 10, 2009; (2009) gln049v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Csiszar, N. Labinskyy, V. Perez, F. A. Recchia, A. Podlutsky, P. Mukhopadhyay, G. Losonczy, P. Pacher, S. N. Austad, A. Bartke, et al. Endothelial function and vascular oxidative stress in long-lived GH/IGF-deficient Ames dwarf mice Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H1882 - H1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Ryan, H. J. Dudash, M. Docherty, K. B. Geronilla, B. A. Baker, G. G. Haff, R. G. Cutlip, and S. E. Alway Aging-Dependent Regulation of Antioxidant Enzymes and Redox Status in Chronically Loaded Rat Dorsiflexor Muscles J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2008; 63(10): 1015 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Csiszar, M. Wang, E. G. Lakatta, and Z. Ungvari Inflammation and endothelial dysfunction during aging: role of NF-{kappa}B J Appl Physiol, October 1, 2008; 105(4): 1333 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Csiszar, N. Labinskyy, A. Podlutsky, P. M. Kaminski, M. S. Wolin, C. Zhang, P. Mukhopadhyay, P. Pacher, F. Hu, R. de Cabo, et al. Vasoprotective effects of resveratrol and SIRT1: attenuation of cigarette smoke-induced oxidative stress and proinflammatory phenotypic alterations Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2721 - H2735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ungvari, N. Labinskyy, S. Gupte, P. N. Chander, J. G. Edwards, and A. Csiszar Dysregulation of mitochondrial biogenesis in vascular endothelial and smooth muscle cells of aged rats Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2121 - H2128. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
