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Am J Physiol Heart Circ Physiol 283: H220-H226, 2002. First published March 7, 2002; doi:10.1152/ajpheart.00950.2001
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Vol. 283, Issue 1, H220-H226, July 2002

Ovariectomy upregulates expression of estrogen receptors, NOS, and HSPs in porcine platelets

Muthuvel Jayachandran and Virginia M. Miller

Department of Surgery and Physiology and Biophysics, Mayo Clinic and Foundation, Rochester, Minnesota 55905

Platelets participate in normal and pathological thrombotic processes. Hormone replacement in postmenopausal women is associated with increase risk for thrombosis. However, little is known regarding how platelets are affected by hormonal status. Nitric oxide (NO) modulates platelet functions and is modulated by hormones. Therefore, the present study was designed to determine how loss of ovarian hormones changes expression of estrogen receptors and regulatory proteins for NO synthase (NOS) in platelets. Estrogen receptors (ERalpha and ERbeta ), NOS, heat shock proteins 70 and 90 (HSP70 and HSP90), caveolin-1, -2, and -3, calmodulin, NOS activity, and cGMP were analyzed in a lysate of platelets from gonadally intact and ovariectomized female pigs. Expression of ERbeta and ERalpha receptors, endothelial NOS (eNOS), HSP70, and HSP90 increased with ovariectomy. NOS activity and cGMP also increased; calmodulin was unchanged. Caveolins were not detected. These results suggest that ovarian hormones influence expression of estrogen receptors and eNOS in platelets. Changes in estrogen receptors and NOS could affect platelet aggregation in response to hormone replacement.

17beta -estradiol; hormones; nitric oxide synthase; endothelial nitric oxide synthase; megakaryocytes; inducible nitric oxide synthase; heat shock protein


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