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Am J Physiol Heart Circ Physiol 283: H227-H237, 2002. First published March 28, 2002; doi:10.1152/ajpheart.00978.2001
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Vol. 283, Issue 1, H227-H237, July 2002

Expression of kinin B1 receptor in fresh or cultured rabbit aortic smooth muscle: role of NF-kappa B

Thierry Sabourin1, Guillaume Morissette1, Johanne Bouthillier1, Luc Levesque2, and François Marceau1

1 Centre Hospitalier Universitaire de Québec, Centre de Recherche du Pavillon l'Hôtel-Dieu de Québec, Québec G1R 2J6; and 2 Angiogene Incorporated, Montréal, Québec, Canada H2L 4M1

Kinin B1 receptor (B1R) expression and the importance of the transcription factor nuclear factor (NF)-kappa B in this process were evaluated in models based on the rabbit aorta: freshly isolated tissue (postisolation induction) and cultured smooth muscle cells (SMCs). A 3-h incubation of freshly isolated tissues determined a sharp B1R mRNA increase (RT-PCR). Coincubation of tissues with a stimulus (interleukin-1beta , fetal bovine serum, epidermal growth factor, or cycloheximide) further increased mRNA levels. Cultured SMCs possessed a basal population of surface B1Rs ([3H]Lys-des-Arg9-bradykinin binding) that was upregulated by treatments with the same set of stimuli (binding, mRNA, nuclear runon). Pharmacological inhibitors of NF-kappa B (MG-132, BAY 11-7082, dexamethasone) or actinomycin D reduced the postisolation induction of B1Rs in fresh aortic tissue (contractility or mRNA) and the cytokine effect on cells (mRNA, binding). NF-kappa B may be a common mediator of various stimuli that increase B1R gene transcription in the rabbit aorta, including tissue isolation, but cycloheximide also stabilizes B1R mRNA. The SMC models faithfully mimic the in vivo situation with regard to B1R regulation.

rabbit aortic smooth muscle cells; nuclear factor-kappa B; interleukin-1; epidermal growth factor


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