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Am J Physiol Heart Circ Physiol 283: H1225-H1236, 2002. First published May 16, 2002; doi:10.1152/ajpheart.00162.2001
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Vol. 283, Issue 3, H1225-H1236, September 2002

Normal contractions triggered by ICa,L in ventricular myocytes from rats with postinfarction CHF

Ivar Sjaastad1,2, Janny Bøkenes1, Fredrik Swift1, J. Andrew Wasserstrom3, and Ole M. Sejersted1

1 Institute for Experimental Medical Research, University of Oslo; 2 Department of Cardiology, Heart and Lung Center, Ullevaal University Hospital, 0407 Oslo, Norway; and 3 Cardiology Division, Department of Medicine, and the Feinberg Cardiovascular Research Institute, Northwestern University Medical School, Chicago, Illinois 60611

Attenuated L-type Ca2+ current (ICa,L), or current-contraction gain have been proposed to explain impaired cardiac contractility in congestive heart failure (CHF). Six weeks after coronary artery ligation, which induced CHF, left ventricular myocytes from isoflurane-anesthetized rats were current or voltage clamped from -70 mV. In both cases, contraction and contractility were attenuated in CHF cells compared with cells from sham-operated rats when cells were only minimally dialyzed using high-resistance microelectrodes. With patch pipettes, cell dialysis caused attenuation of contractions in sham cells, but not CHF cells. Stepping from -50 mV, the following variables were not different between sham and CHF, respectively: peak ICa,L (4.5 ± 0.3 vs. 3.8 ± 0.3 pApF-1 at 23°C and 9.4 ± 0.5 vs. 8.4 ± 0.5 pApF-1 at 37°C), the bell-shaped voltage-contraction relationship in Cs+ solutions (fractional shortening, 15.2 ± 1.0% vs. 14.3 ± 0.7%, respectively, at 23°C and 7.5 ± 0.4% vs. 6.7 ± 0.5% at 37°C) and the sigmoidal voltage-contraction relationship in K+ solutions. Caffeine-induced Ca2+ release and sarcoplasmic reticulum Ca2+-ATPase-to-phospholamban ratio were not different. Thus CHF contractions triggered by ICa,L were normal, and the contractile deficit was only seen in undialyzed cardiomyocytes stimulated from -70 mV.

electrophysiology; myocardial infarction; sarcoplasmic reticulum Ca2+ ATPase; phospholamban; caffeine; L-type Ca2+ current; congestive heart failure


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