AJP - Heart Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H1282-H1291, 2002. First published June 13, 2002; doi:10.1152/ajpheart.00117.2002
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/4/H1282    most recent
00117.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mulivor, A. W.
Right arrow Articles by Lipowsky, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mulivor, A. W.
Right arrow Articles by Lipowsky, H. H.
Vol. 283, Issue 4, H1282-H1291, October 2002

Role of glycocalyx in leukocyte-endothelial cell adhesion

A. W. Mulivor and H. H. Lipowsky

Department of Bioengineering, Pennsylvania State University, University Park, Pennsylvania 16802

The binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-µm length of venule after superfusion of the mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC) adhesion. Removal of the EC glycocalyx by micropipette infusion of the venule with heparinase increased FLM-EC adhesion to levels attained with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites. Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC adhesion fell because of possible removal of selectins necessary to maintain WBC rolling at the wall. It is concluded that the glycocalyx serves as a barrier to adhesion and that its shedding during natural activation of ECs may be an essential part of the inflammatory response.

endothelium; heparinase; N-formylmethionyl-leucyl-phenylalanine


This article has been cited by other articles:


Home page
 Circ. Res.Home page
D. Chappell, M. Jacob, O. Paul, M. Rehm, U. Welsch, M. Stoeckelhuber, P. Conzen, and B. F. Becker
The Glycocalyx of the Human Umbilical Vein Endothelial Cell: An Impressive Structure Ex Vivo but Not in Culture
Circ. Res., June 5, 2009; 104(11): 1313 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 PerfusionHome page
K Svennevig, T. Hoel, A. Thiara, S. Kolset, A Castelheim, T. Mollnes, F Brosstad, E Fosse, and J. Svennevig
Syndecan-1 plasma levels during coronary artery bypass surgery with and without cardiopulmonary bypass
Perfusion, May 1, 2008; 23(3): 165 - 171.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
M. Nieuwdorp, M. C. Meuwese, H. L. Mooij, C. Ince, L. N. Broekhuizen, J. J. P. Kastelein, E. S. G. Stroes, and H. Vink
Measuring endothelial glycocalyx dimensions in humans: a potential novel tool to monitor vascular vulnerability
J Appl Physiol, March 1, 2008; 104(3): 845 - 852.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. P. Stevens, V. Hlady, and R. O. Dull
Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L328 - L335.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
J. J. Bergan, G. W. Schmid-Schonbein, P. D. C. Smith, A. N. Nicolaides, M. R. Boisseau, and B. Eklof
Chronic Venous Disease
N. Engl. J. Med., August 3, 2006; 355(5): 488 - 498.
[Full Text] [PDF]

Home page
 J. Physiol.Home page
R. Kelly, T. Ruane-O'Hora, M. I. M. Noble, A. J. Drake-Holland, and H. M. Snow
Differential inhibition by hyperglycaemia of shear stress- but not acetylcholine-mediated dilatation in the iliac artery of the anaesthetized pig
J. Physiol., May 15, 2006; 573(1): 133 - 145.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Mehta and A. B. Malik
Signaling Mechanisms Regulating Endothelial Permeability
Physiol Rev, January 1, 2006; 86(1): 279 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Gouverneur, J. A. E. Spaan, H. Pannekoek, R. D. Fontijn, and H. Vink
Fluid shear stress stimulates incorporation of hyaluronan into endothelial cell glycocalyx
Am J Physiol Heart Circ Physiol, January 1, 2006; 290(1): H458 - H452.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
Y. Banz, O. M. Hess, S. C. Robson, D. Mettler, P. Meier, A. Haeberli, E. Csizmadia, E. Y. Korchagina, N. V. Bovin, and R. Rieben
Locally targeted cytoprotection with dextran sulfate attenuates experimental porcine myocardial ischaemia/reperfusion injury
Eur. Heart J., November 1, 2005; 26(21): 2334 - 2343.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. J. Zuurbier, C. Demirci, A. Koeman, H. Vink, and C. Ince
Short-term hyperglycemia increases endothelial glycocalyx permeability and acutely decreases lineal density of capillaries with flowing red blood cells
J Appl Physiol, October 1, 2005; 99(4): 1471 - 1476.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Kim, A. S. Popel, M. Intaglietta, and P. C. Johnson
Aggregate formation of erythrocytes in postcapillary venules
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H584 - H590.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. M. Ainslie, J. S. Garanich, R. O. Dull, and J. M. Tarbell
Vascular smooth muscle cell glycocalyx influences shear stress-mediated contractile response
J Appl Physiol, January 1, 2005; 98(1): 242 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. W. Mulivor and H. H. Lipowsky
Inflammation- and ischemia-induced shedding of venular glycocalyx
Am J Physiol Heart Circ Physiol, May 1, 2004; 286(5): H1672 - H1680.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. H. Platts and B. R. Duling
Adenosine A3 Receptor Activation Modulates the Capillary Endothelial Glycocalyx
Circ. Res., January 9, 2004; 94(1): 77 - 82.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. Netelenbos, J. van den Born, F. L. Kessler, S. Zweegman, P. C. Huijgens, and A. M. Drager
In vitro model for hematopoietic progenitor cell homing reveals endothelial heparan sulfate proteoglycans as direct adhesive ligands
J. Leukoc. Biol., December 1, 2003; 74(6): 1035 - 1044.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
J. A. Florian, J. R. Kosky, K. Ainslie, Z. Pang, R. O. Dull, and J. M. Tarbell
Heparan Sulfate Proteoglycan Is a Mechanosensor on Endothelial Cells
Circ. Res., November 14, 2003; 93 (10): e136 - e142.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. A. Constantinescu, H. Vink, and J. A.E. Spaan
Endothelial Cell Glycocalyx Modulates Immobilization of Leukocytes at the Endothelial Surface
Arterioscler Thromb Vasc Biol, September 1, 2003; 23(9): 1541 - 1547.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. H. Platts, J. Linden, and B. R. Duling
Rapid modification of the glycocalyx caused by ischemia-reperfusion is inhibited by adenosine A2A receptor activation
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2360 - H2367.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online