AJP - Heart Calcium Transients and Cell-Sarcomere
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H1811-H1818, 2002. First published July 11, 2002; doi:10.1152/ajpheart.01092.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/5/H1811    most recent
01092.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, J. M.
Right arrow Articles by De Potter, W. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, J. M.
Right arrow Articles by De Potter, W. P.
Vol. 283, Issue 5, H1811-H1818, November 2002

Presence of cellular renin-angiotensin system in chromaffin cells of bovine adrenal medulla

Jun Ming Wang1,2, Dirk Slembrouck1, Junhui Tan2, Lut Arckens3, Frans H. H. Leenen2, Pierre J. Courtoy4, and Werner P. De Potter1

1 Laboratory of Neuropharmacology and Neurobiology, Department of Medicine, Universitaire Instelling Antwerpen, University of Antwerp, B-2610 Antwerp, Belgium; 2 Hypertension Unit, University of Ottawa Heart Institute, Ottawa, Ontario K1Y 4W7, Canada; 3 Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Katholieke Universiteit Leuven, B-3000 Louvain, Belgium; 4 Cell Biology Unit, Louvain University Medical School, B-1200 Brussels, Belgium

The presence of a local renin-angiotensin system has been established in organs that serve as angiotensin targets. In this study, the expression of angiotensinogen mRNA and subcellular localization of renin, angiotensin-converting enzyme, and angiotensin II were investigated in bovine adrenal medullary cells in primary culture. By light microscopy, expression of angiotensinogen mRNA, immunoreactive renin, angiotensin-converting enzyme, and angiotensin II were readily detectable only in the chromaffin cells. The density distribution of renin and angiotensin II in sucrose gradients suggested a concentration in chromaffin granules, a localization directly confirmed by immunoelectron microscopy. Reverse transcriptase-polymerase chain reaction and sequencing confirmed the expression of angiotensinogen in bovine chromaffin cells and the adrenal medulla. In addition, in vitro autoradiography indicated that both angiotensin-converting enzyme and angiotensin type 1 receptors were present in the adrenal medulla. These results provide the first direct evidence that chromaffin cells in the adrenal medulla are not only the target for angiotensin but should also be considered as potential local angiotensin-generating and -storing cells.

in situ hybridization; immunocytochemistry; radioligand autoradiography; angiotensinogen


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
T. M. Gwathmey, H. A. Shaltout, K. D. Pendergrass, N. T. Pirro, J. P. Figueroa, J. C. Rose, D. I. Diz, and M. C. Chappell
Nuclear angiotensin II type 2 (AT2) receptors are functionally linked to nitric oxide production
Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1484 - F1493.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online