AJP - Heart Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H2102-H2109, 2002. First published July 11, 2002; doi:10.1152/ajpheart.01071.2001
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A corrigendum has been published
Right arrow All Versions of this Article:
283/5/H2102    most recent
01071.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baetz, D.
Right arrow Articles by Feuvray, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baetz, D.
Right arrow Articles by Feuvray, D.
Vol. 283, Issue 5, H2102-H2109, November 2002

The ERK pathway regulates Na+-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> cotransport activity in adult rat cardiomyocytes

Delphine Baetz1, Robert S. Haworth2, Metin Avkiran2, and Danielle Feuvray1

1 Laboratoire de Physiologie Cellulaire and Centre National de la Recherche Scientifique, Hôpital Marie Lannelongue-Université Paris XI, 91405 Orsay Cedex, France; and 2 Centre for Cardiovascular Biology and Medicine, King's College London, The Rayne Institute, St. Thomas' Hospital, London 7E1 7EH, United Kingdom

The sarcolemmal Na+-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> cotransporter (NBC) is stimulated by intracellular acidification and acts as an acid extruder. We examined the role of the ERK pathway of the MAPK cascade as a potential mediator of NBC activation by intracellular acidification in the presence and absence of angiotensin II (ANG II) in adult rat ventricular myocytes. Intracellular pH (pHi) was recorded with the use of seminaphthorhodafluor-1. The NH<UP><SUB>4</SUB><SUP>+</SUP></UP> method was used to induce an intracellular acid load. NBC activation was significantly decreased with the ERK inhibitors PD-98059 and U-0126. NBC activity after acidification was increased in the presence of ANG II (pHi range of 6.75-7.00). ANG II plus PD-123319 (AT2 antagonist) still increased NBC activity, whereas ANG II plus losartan (AT1 antagonist) did not affect it. ERK phosphorylation (measured by immunoblot analysis) during intracellular acidification was increased by ANG II, an effect that was abolished by losartan and U-0126. In conclusion, the MAPK(ERK)-dependent pathway facilitates the rate of pHi recovery from acid load through NBC activity and is involved in the AT1 receptor-mediated stimulation of such activity by ANG II.

cardiac ventricular myocytes; angiotensin II


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Perry, J. Blaine, H. Le, and I. I. Grichtchenko
PMA- and ANG II-induced PKC regulation of the renal Na+-HCO3- cotransporter (hkNBCe1)
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F417 - F427.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Avkiran and R. S Haworth
Regulatory effects of G protein-coupled receptors on cardiac sarcolemmal Na+/H+ exchanger activity: signalling and significance
Cardiovasc Res, March 15, 2003; 57(4): 942 - 952.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online