AJP - Heart Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 283: H2379-H2388, 2002. First published August 8, 2002; doi:10.1152/ajpheart.00410.2002
0363-6135/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/6/H2379    most recent
00410.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, G.
Right arrow Articles by Cooper, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, G.
Right arrow Articles by Cooper, G., IV
Vol. 283, Issue 6, H2379-H2388, December 2002

Inhibition of G protein-coupled receptor trafficking in neuroblastoma cells by MAP 4 decoration of microtubules

Guangmao Cheng, Yoshihiro Iijima, Yuji Ishibashi, Dhandapani Kuppuswamy, and George Cooper IV

Cardiology Division, Gazes Cardiac Research Institute, Medical University of South Carolina, and Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401

One mechanism for the reappearance of G protein-coupled receptors after agonist activation is microtubule-based transport. In pressure-overload cardiac hypertrophy, there is downregulation of G protein-coupled receptors and the appearance of a densified microtubule network extensively decorated by a microtubule-associated protein, MAP 4. Our hypothesis is that overdecoration of a dense microtubule network with this structural protein, as in hypertrophied myocardium, would impede receptor recovery. We tested this hypothesis by studying muscarinic acetylcholine receptor (mAChR) internalization and recovery after agonist stimulation in neuroblastoma cells. Exposure of cells to carbachol, a muscarinic receptor agonist, decreased membrane receptor binding activity. After carbachol withdrawal, receptor binding recovered toward the initial value. When microtubules were depolymerized before carbachol withdrawal, mAChR recovery was only 44% of that in intact cells. Cells were then infected with an adenovirus containing MAP 4 cDNA. MAP 4 protein decorated the microtubules extensively, and receptor recovery upon carbachol withdrawal was reduced to 54% of control. Thus muscarinic receptor recovery after agonist exposure is microtubule dependent, and MAP 4 decoration of microtubules inhibits receptor recovery.

muscarinic receptors; microtubule-associated proteins; gene transfer techniques; radioligand assay


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Scholz, C. F. Baicu, W. J. Tuxworth, L. Xu, H. Kasiganesan, D. R. Menick, and G. Cooper IV
Microtubule-dependent distribution of mRNA in adult cardiocytes
Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1135 - H1144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Cooper IV
Cytoskeletal networks and the regulation of cardiac contractility: microtubules, hypertrophy, and cardiac dysfunction
Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1003 - H1014.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
D. Scholz, P. McDermott, M. Garnovskaya, T. N. Gallien, S. Huettelmaier, C. DeRienzo, and G. Cooper IV
Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes
Cardiovasc Res, August 1, 2006; 71(3): 506 - 516.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Cheng, F. Qiao, T. N. Gallien, D. Kuppuswamy, and G. Cooper IV
Inhibition of {beta}-adrenergic receptor trafficking in adult cardiocytes by MAP4 decoration of microtubules
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1193 - H1202.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S.R. Bailey, A.H. Eid, S. Mitra, S. Flavahan, and N.A. Flavahan
Rho Kinase Mediates Cold-Induced Constriction of Cutaneous Arteries: Role of {alpha}2C-Adrenoceptor Translocation
Circ. Res., May 28, 2004; 94(10): 1367 - 1374.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online