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Division of Endocrinology and Department of Medicine, North Shore University Hospital/New York University School of Medicine, Manhasset 11030; and Department of Cell Biology, New York University School of Medicine, New York, New York 10021
We developed an RT-PCR assay to study
both the time course and the mechanism for the triiodothyronine
(T3)-induced transcription of the 
- and 
-myosin heavy
chain (MHC) genes in vivo on the basis of the quantity of specific
heterogeneous nuclear RNA (hnRNA). The temporal relationship of changes
in transcriptional activity to the amount of -MHC mRNA and the
coordinated regulation of transcription of more than one gene in
response to T3 are demonstrated here for the first time.
Quantitation of -MHC hnRNA demonstrated that T3 induced
-MHC transcription in hypothyroid rats within 30 min of a single
injection of T3 (0.5 µg/100 g body wt). Maximal transcription rates (135% ± 15.8 of euthyroid values) occurred 6 h after injection and subsequently declined in parallel with serum
T3 levels. The transcription of -MHC was reduced to 86% of peak hypothyroid levels 6 h after a single T3
injection and reached a nadir of 59% of hypothyroid levels at 36 h. Analysis of the time course of T3-mediated induction of
-MHC hnRNA and repression of -MHC hnRNA indicates that separate
molecular mechanisms are involved in the coordinated regulation of
these genes.
cardiac contractility; heterogeneous nuclear ribonucleic acid; thyroid hormone; thyroid disease
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