AJP - Heart Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Heart Circ Physiol 284: H2311-H2319, 2003. First published January 30, 2003; doi:10.1152/ajpheart.00932.2001
0363-6135/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/6/H2311    most recent
00932.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, J.-C.
Right arrow Articles by Dinh-Xuan, A. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, J.-C.
Right arrow Articles by Dinh-Xuan, A. T.
Vol. 284, Issue 6, H2311-H2319, June 2003

Involvement of Ca2+/calmodulin-dependent protein kinase II in endothelial NO production and endothelium-dependent relaxation

Jean-Christophe Schneider1, Driss El Kebir1, Christiane Chéreau2, Sophie Lanone3, Xiao-Lin Huang1, Anthony S. De Buys Roessingh1, Jean-Christophe Mercier4, Josette Dall'Ava-Santucci1, and A. Tuan Dinh-Xuan1

1 Service de Physiologie-Explorations Fonctionnelles, Centre Hospitalier Universitaire Cochin, Assistance Publique, Hôpitaux de Paris, Université Paris 5, 75014 Paris; 2 Laboratoire d'Immunologie Biologique, Faculté de Médecine Cochin, Université Paris 5, 75014 Paris; 3 Unité 408, Institut National de la Santé et de la Recherche Médicale, 75018 Paris; and 4 Service de Réanimation Pédiatrique, Hôpital Robert Debré, Assistance Publique, Hôpitaux de Paris, 75019 Paris, France

Nitric oxide (NO) is synthesized from L-arginine by the Ca2+/calmodulin-sensitive endothelial NO synthase (NOS) isoform (eNOS). The present study assesses the role of Ca2+/calmodulin-dependent protein kinase II (CaMK II) in endothelium-dependent relaxation and NO synthesis. The effects of three CaMK II inhibitors were investigated in endothelium-intact aortic rings of normotensive rats. NO synthesis was assessed by a NO sensor and chemiluminescence in culture medium of cultured porcine aortic endothelial cells stimulated with the Ca2+ ionophore A23187 and thapsigargin. Rat aortic endothelial NOS activity was measured by the conversion of L-[3H]arginine to L-[3H]citrulline. Three CaMK II inhibitors, polypeptide 281-302, KN-93, and lavendustin C, attenuated the endothelium-dependent relaxation of endothelium-intact rat aortic rings in response to acetylcholine, A23187, and thapsigargin. None of the CaMK II inhibitors affected the relaxation induced by NO donors. In a porcine aortic endothelial cell line, KN-93 decreased NO synthesis and caused a rightward shift of the concentration-response curves to A23187 and thapsigargin. In rat aortic endothelial cells, KN-93 significantly decreased bradykinin-induced eNOS activity. These results suggest that CaMK II was involved in NO synthesis as a result of Ca2+-dependent activation of eNOS.

endothelial function; nitric oxide; protein phosphorylation; signal transduction; thapsigargin


This article has been cited by other articles:


Home page
 CirculationHome page
C. G. da Silva, A. Specht, B. Wegiel, C. Ferran, and E. Kaczmarek
Mechanism of Purinergic Activation of Endothelial Nitric Oxide Synthase in Endothelial Cells
Circulation, February 17, 2009; 119(6): 871 - 879.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
D. Maiti, Z. Xu, and E. J. Duh
Vascular Endothelial Growth Factor Induces MEF2C and MEF2-Dependent Activity in Endothelial Cells
Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3640 - 3648.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. Cai, D. Liu, and J. G.N. Garcia
CaM Kinase II-dependent pathophysiological signalling in endothelial cells
Cardiovasc Res, January 1, 2008; 77(1): 30 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. G. Soucy, S. Ryoo, A. Benjo, H. K. Lim, G. Gupta, J. S. Sohi, J. Elser, M. A. Aon, D. Nyhan, A. A. Shoukas, et al.
Impaired shear stress-induced nitric oxide production through decreased NOS phosphorylation contributes to age-related vascular stiffness
J Appl Physiol, December 1, 2006; 101(6): 1751 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. W. Leffler, L. Balabanova, A. L. Fedinec, and H. Parfenova
Nitric oxide increases carbon monoxide production by piglet cerebral microvessels
Am J Physiol Heart Circ Physiol, October 1, 2005; 289(4): H1442 - H1447.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
V. Saraswathi, G. Wu, M. Toborek, and B. Hennig
Linoleic acid-induced endothelial activation: role of calcium and peroxynitrite signaling
J. Lipid Res., May 1, 2004; 45(5): 794 - 804.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.