| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of 1Pharmacology and 2Integrative Physiology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan
Submitted 10 December 2002 ; accepted in final form 19 March 2003
The measurement of hemoglobin-nitric oxide (NO) adduct (HbNO) in whole
blood by the electron paramagnetic resonance (EPR) method seems relevant for
the assessment of systemic NO levels. However, ceruloplasmin and unknown
radical species overlap the same magnetic field as that of HbNO. To reveal the
EPR spectrum of HbNO, we then introduced the EPR signal subtraction method,
which is based on the computer-assisted subtraction of the digitized EPR
spectrum of HbNO-depleted blood from that of sample blood using the software.
Rats were treated with
N
-nitro-L-arginine methyl ester
(L-NAME; 120 mg · kg–1 ·
day–1) for 1 wk to obtain HbNO-depleted blood.
When this method was applied to the analysis of untreated fresh whole blood,
the five-coordinate state of HbNO was observed. HbNO concentration in
pentobarbital-anesthetized rats was augmented (change in [HbNO] =
1.6–5.5 µM) by infusion of L-arginine (0.2–0.6 g/kg)
but not D-arginine. Using this method, we attempted to evaluate the
effects of temocapril on HbNO dynamics in an L-NAME-induced rat
endothelial dysfunction model. The oral administration of L-NAME
for 2 wk induced a serious hypertension, and the HbNO concentration was
reduced (change in [HbNO] = 5.7 µM). Coadministration of temocapril dose
dependently improved both changes in blood pressure and the systemic HbNO
concentration. In this study, we succeeded in measuring the blood HbNO level
as an index of NO by the EPR HbNO signal subtraction method. We also
demonstrated that temocapril improves abnormalities of NO dynamics in
L-NAME-induced endothelial dysfunction rats using the EPR HbNO
signal subtraction method.
nitric oxide; hemoglobin-nitric oxide adduct; electron paramagnetic resonance; temocapril
This article has been cited by other articles:
|
|
 |
|
  Y. Kanematsu, K. Yamaguchi, H. Ohnishi, Y. Motobayashi, K. Ishizawa, Y. Izawa, K. Kawazoe, S. Kondo, S. Kagami, S. Tomita, et al. Dietary doses of nitrite restore circulating nitric oxide level and improve renal injury in L-NAME-induced hypertensive rats Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1457 - F1462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Derakhshan, G. Hao, and S. S. Gross Balancing reactivity against selectivity: The evolution of protein S-nitrosylation as an effector of cell signaling by nitric oxide Cardiovasc Res, July 15, 2007; 75(2): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Tsuchiya, Y. Kanematsu, M. Yoshizumi, H. Ohnishi, K. Kirima, Y. Izawa, M. Shikishima, T. Ishida, S. Kondo, S. Kagami, et al. Nitrite is an alternative source of NO in vivo Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2163 - H2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Okamoto, K. Tsuchiya, Y. Kanematsu, Y. Izawa, M. Yoshizumi, S. Kagawa, and T. Tamaki Nitrite-derived nitric oxide formation following ischemia-reperfusion injury in kidney Am J Physiol Renal Physiol, January 1, 2005; 288(1): F182 - F187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Foster, J. R. Pawloski, D. J. Singel, and J. S. Stamler Role of Circulating S-Nitrosothiols in Control of Blood Pressure Hypertension, January 1, 2005; 45(1): 15 - 17. [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Stamler S-Nitrosothiols in the Blood: Roles, Amounts, and Methods of Analysis Circ. Res., March 5, 2004; 94(4): 414 - 417. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
