HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/1/H251    most recent 01136.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (18) Citing Articles via Google Scholar Google Scholar Articles by Joaquim, L. F. Articles by Morris, M. Search for Related Content PubMed PubMed Citation Articles by Joaquim, L. F. Articles by Morris, M.

Enhanced heart rate variability and baroreflex index after stress and cholinesterase inhibition in mice

¹Wright State University School of Medicine, Department of Pharmacology and Toxicology, Dayton, Ohio 45435; ²University of Sao Paulo Faculty of Medicine, Ribeirão Preto, Brazil 14049-900; and ³Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic 81371

Submitted 3 December 2003 ; accepted in final form 10 February 2004

Experiments tested the effect of stress coupled with cholinesterase inhibition on blood pressure, heart rate, baroreflex index, and variability in time and frequency domain in conscious mice. The objective was to determine whether cholinergic systems interact with stress to alter cardiovascular responses. Male C57BL/6J mice with arterial catheters were exposed to 3-day treatments: 1) intermittent shaker stress, 2) pyridostigmine (10 mg·kg^–1·day^–1); or 3) combined pyridostigmine and stress. Pyridostigmine reduced blood cholinesterase (–33%) with no added effects of stress. Twenty-four-hour blood pressure recordings showed that there were no differences in blood pressure and heart rate with the treatments. Pulse interval standard deviation was greatly increased in the pyridostigmine/stress group compared with stress or pyridostigmine groups (11.0 ± 1.4, 5.0 ± 0.9, and 7.5 ± 0.9 ms, respectively). Spectral analysis showed two distinct components for pulse interval variability (low and high frequency). Variability in the low-frequency range was greatly enhanced in the pyridostigmine/stress group, seen as a doubling of the power (9.5 ± 1.7, 3.3 ± 0.9, and 5.0 ± 0.6 ms for pyridostigmine/stress, stress and pyridostigmine groups, respectively). Baroreflex sensitivity was also increased in the pyridostigmine/stress group (3.6 ± 0.5 compared with 1.8 ± 0.3 and 1.7 ± 0.5 ms/mmHg in the stress and pyridostigmine groups, respectively). There was no difference in blood pressure variability or its spectral components. Results demonstrate that there are potent interactions between a mild stressor and cholinesterase inhibition seen as an accentuation of low-frequency variability in pulse interval time series, probably associated with baroreflex input and autonomic drive.

cardiovascular; spectral analysis; cholinergic; stress biology

This article has been cited by other articles:

				J. Thireau, B. L. Zhang, D. Poisson, and D. Babuty Heart rate variability in mice: a theoretical and practical guide Exp Physiol, January 1, 2008; 93(1): 83 - 94. [Abstract] [Full Text] [PDF]

				D. Laude, V. Baudrie, and J.-L. Elghozi Applicability of recent methods used to estimate spontaneous baroreflex sensitivity to resting mice Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R142 - R150. [Abstract] [Full Text] [PDF]

				V. Farah, K. M. Elased, and M. Morris Genetic and dietary interactions: role of angiotensin AT1a receptors in response to a high-fructose diet Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1083 - H1089. [Abstract] [Full Text] [PDF]

				V. Baudrie, D. Laude, and J.-L. Elghozi Optimal frequency ranges for extracting information on cardiovascular autonomic control from the blood pressure and pulse interval spectrograms in mice Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R904 - R912. [Abstract] [Full Text] [PDF]

				R. Fazan Jr., M. de Oliveira, V. J. Dias da Silva, L. F. Joaquim, N. Montano, A. Porta, M. W. Chapleau, and H. C. Salgado Frequency-dependent baroreflex modulation of blood pressure and heart rate variability in conscious mice Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H1968 - H1975. [Abstract] [Full Text] [PDF]

				Y. Chen, H. Chen, and M. Morris Enhanced osmotic responsiveness in angiotensin AT1a receptor deficient mice: evidence for a role for AT1b receptors Exp Physiol, September 1, 2005; 90(5): 739 - 746. [Abstract] [Full Text] [PDF]

				S. R. Raj, B. K. Black, I. Biaggioni, P. A. Harris, and D. Robertson Acetylcholinesterase Inhibition Improves Tachycardia in Postural Tachycardia Syndrome Circulation, May 31, 2005; 111(21): 2734 - 2740. [Abstract] [Full Text] [PDF]

				Y. Chen, L. F. Joaquim, V. M. Farah, R. B. Wichi, R. Fazan Jr., H. C. Salgado, and M. Morris Cardiovascular autonomic control in mice lacking angiotensin AT1a receptors Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R1071 - R1077. [Abstract] [Full Text] [PDF]