HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/3/H994    most recent 00400.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Ueda, A. Articles by Tanishita, K. Search for Related Content PubMed PubMed Citation Articles by Ueda, A. Articles by Tanishita, K.

Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model

¹School of Fundamental Science and Technology, ²Department of System Design Engineering, and ³Keio Leading-Edge Laboratory of Science and Technology, Keio University, Yokohama 223-8522; and ⁴Department of Mechanical Engineering, Shibaura Institute of Technology, Tokyo 108-8548, Japan

Submitted 2 May 2003 ; accepted in final form 29 April 2004

Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this model in detail using phase-contrast microscopy, confocal laser scanning microscopy, and electron microscopy. The results show that cells invaded the collagen gel and reconstructed the tubular structures, containing a clearly defined lumen consisting of multiple cells. The model was placed in a parallel-plate flow chamber. A laminar shear stress of 0.3 Pa was applied to the surfaces of the cells for 48 h. Promotion of microvessel network formation was detectable after 10 h in the flow chamber. After 48 h, the length of networks exposed to shear stress was 6.17 (±0.59) times longer than at the initial state, whereas the length of networks not exposed to shear stress was only 3.30 (±0.41) times longer. The number of bifurcations and endpoints increased for networks exposed to shear stress, whereas the number of bifurcations alone increased for networks not exposed to shear stress. These results demonstrate that shear stress applied to the surfaces of endothelial cells on collagen gel promotes the growth of microvessel network formation in the gel and expands the network because of repeated bifurcation and elongation.

angiogenesis; blood flow; capillary; in vitro model; migration

This article has been cited by other articles:

				H. Kang, K. J. Bayless, and R. Kaunas Fluid shear stress modulates endothelial cell invasion into three-dimensional collagen matrices Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H2087 - H2097. [Abstract] [Full Text] [PDF]

				W. Zheng, L. P. Christensen, and R. J. Tomanek Differential effects of cyclic and static stretch on coronary microvascular endothelial cell receptors and vasculogenic/angiogenic responses Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H794 - H800. [Abstract] [Full Text] [PDF]

				S. L. Tressel, R.-P. Huang, N. Tomsen, and H. Jo Laminar Shear Inhibits Tubule Formation and Migration of Endothelial Cells by an Angiopoietin-2-Dependent Mechanism Arterioscler Thromb Vasc Biol, October 1, 2007; 27(10): 2150 - 2156. [Abstract] [Full Text] [PDF]

				A. J. Hwa, R. C. Fry, A. Sivaraman, P. T. So, L. D. Samson, D. B. Stolz, and L. G. Griffith Rat liver sinusoidal endothelial cells survive without exogenous VEGF in 3D perfused co-cultures with hepatocytes FASEB J, August 1, 2007; 21(10): 2564 - 2579. [Abstract] [Full Text] [PDF]