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This Article Full Text Full Text (PDF) All Versions of this Article: 288/5/H2146    most recent 00749.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Cabrales, P. Articles by Intaglietta, M. Search for Related Content PubMed PubMed Citation Articles by Cabrales, P. Articles by Intaglietta, M.

Effects of extreme hemodilution with hemoglobin-based O₂ carriers on microvascular pressure

Department of Bioengineering, University of California-San Diego, La Jolla, California

Submitted 27 July 2004 ; accepted in final form 30 December 2004

A surface-modified polyethylene glycol-conjugated human hemoglobin (MP4) and -cross-linked human hemoglobin (Hb) were used to restore oxygen carrying capacity in conditions of extreme hemodilution (hematocrit 11%) in the hamster window model preparation. Changes in microvascular function were analyzed in terms of effects on capillary pressure and functional capillary density (FCD). MP4, at 1.0 ± 0.2 g/dl blood concentration, significantly lowered mean arterial pressure (MAP) below baseline (99.6 ± 7.6 mmHg) to 82.4 ± 6.9 mmHg (P < 0.05) and decreased of FCD to 70 ± 9%. Hb caused a greater recovery in MAP to 94.4 ± 6.2 mmHg and lowered FCD to 62 ± 8%. However, differences between Hb and MP4 in FCD were not statistically significant. Capillary pressures were in the ranges of 17–21 mmHg for MP4 and 15–19 mmHg for Hb, with both significantly lower than baseline (P < 0.05). Pressure in 80-µm-diameter arterioles was significantly increased with Hb relative to MP4 (P < 0.05). These results were compared with previous findings on the relation between capillary pressure and FCD; they supported the concept of a relationship between FCD and capillary pressure. Measurement of changes in arteriolar diameter, microvascular blood flow, and FCD show that there was no statistical difference between using Hb and MP4 in extreme hemodilution. Microvascular resistance in arterioles with a diameter range of 70–80 µm showed an increase relative to control with Hb, whereas MP4 caused a decrease.

shear stress; perfusion; plasma expander; blood pressure; blood substitutes

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