AJP - Heart Calcium Transients and Cell-Sarcomere
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Am J Physiol Heart Circ Physiol 289: H801-H812, 2005. First published March 25, 2005; doi:10.1152/ajpheart.01227.2004
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Power output is linearly related to MyHC content in rat skinned myocytes and isolated working hearts

F. Steven Korte, Todd J. Herron, Michael J. Rovetto, and Kerry S. McDonald

Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, Missouri

Submitted 6 December 2004 ; accepted in final form 24 March 2005

The amount of work the heart can perform during ejection is governed by the inherent contractile properties of individual myocytes. One way to alter contractile properties is to alter contractile proteins such as myosin heavy chain (MyHC), which is known to demonstrate isoform plasticity in response to disease states. The purpose of this study was to examine myocyte functionality over the complete range of MyHC expression in heart, from 100% {alpha}-MyHC to 100% {beta}-MyHC, using euthyroid and hypothyroid rats. Peak power output in skinned cardiac myocytes decreased as a nearly linear function of {beta}-MyHC expression during maximal (r2 = 0.85, n = 44 myocyte preparations) and submaximal (r2 = 0.82, n = 31 myocyte preparations) Ca2+ activation. To determine whether single myocyte function translated to the level of the whole heart, power output was measured in working heart preparations expressing varied ratios of MyHC. Left ventricular power output of isolated working heart preparations also decreased as a linear function of increasing {beta}-MyHC expression (r2 = 0.82, n = 34 myocyte preparations). These results demonstrate that power output is highly dependent on MyHC expression in single myocytes, and this translates to the performance of working left ventricles.

myosin heavy chain; myocardium; myocardial contractility; cardiac muscle


Address for reprint requests and other correspondence: K. S. McDonald, One Hospital Drive, MA415 Medical Sciences Bldg., Dept. of Medical Pharmacology and Physiology, Columbia, MO 65212 (E-mail: mcdonaldks{at}missouri.edu)




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