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Structural differences in two biochemically defined populations of cardiac mitochondria

¹Department of Cytomorphology, School of Medicine, University of Cagliari, Cagliari, Italy; ²Department of Biological Sciences, School of Dental Medicine, and ³Departments of Pharmacology and Medicine, School of Medicine, Case Western Reserve University and Louis Stokes Veterans Affairs Medical Center, Cleveland, Ohio

Submitted 24 August 2004 ; accepted in final form 22 March 2005

To determine whether there are structural differences in two topologically separated, biochemically defined mitochondrial populations in rat heart myocytes, the interior of these organelles was examined by high-resolution scanning electron microscopy. On the basis of a count of 159 in situ subsarcolemmal mitochondria (SSM, i.e., those that directly abut the sarcolemma), these organelles possess mainly lamelliform cristae (77%), whereas the cristae in in situ interfibrillar mitochondria (IFM, i.e., those situated between the myofibrils, n = 300) are mainly tubular (55%) or a mixture of tubular and lamelliform (24%). Isolated SSM (n = 374), similar to their in situ counterparts, have predominantly lamelliform cristae (75%). The proportions of crista types in isolated IFM (n = 337) have been altered, with only 20% of these organelles retaining exclusively tubular cristae, whereas 58% are mixed; of the latter, lamelliform cristae predominate. This finding suggests that, in contrast to SSM, the cristae in IFM are structurally plastic, changing during isolation. These observations on >1,000 organelles provide the first quantitative morphological evidence for definitive differences between the two populations of cardiac mitochondria.

high-resolution scanning electron microscopy; cardiomyocytes; heart; cristae

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