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This Article Full Text Full Text (PDF) All Versions of this Article: 289/6/H2503    most recent 00587.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by van Haaren, P. M. A. Articles by Spaan, J. A. E. Search for Related Content PubMed PubMed Citation Articles by van Haaren, P. M. A. Articles by Spaan, J. A. E.

Charge modification of the endothelial surface layer modulates the permeability barrier of isolated rat mesenteric small arteries

Department of Medical Physics, Cardiovascular Research Institute Amsterdam, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

Submitted 2 June 2005 ; accepted in final form 25 July 2005

We hypothesized that modulation of the effective charge density of the endothelial surface layer (ESL) results in altered arterial barrier properties to transport of anionic solutes. Rat mesenteric small arteries (diameter 190 µm) were isolated, cannulated, perfused, and superfused with MOPS-buffered physiological salt solutions. MOPS-solutions were of normal ionic strength (162 mM, MOPS), low ionic strength (81 mM, LO-MOPS), or high ionic strength (323 mM, HI-MOPS), to modulate ESL charge density (normal, high, or low ESL charge, respectively). Osmolarity of MOPS, LO-MOPS, and HI-MOPS was kept constant at 297 mosmol/l, using additional glucose when necessary. Perfusate solutions were supplemented with 1% BSA. Arteries were cannulated with a double-barreled theta-pipet on the inlet side and a regular pipet on the outlet side. After infusion of FITC-labeled dextran of 50 kDa (FITC-50) and the endothelial membrane dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, the dynamics of arterial dye filling were determined with confocal microscopy. ESL thickness, as determined from the initial exclusion zone for FITC-50 on the luminal endothelial surface, was 6.3 ± 1.4 µm for LO-MOPS, 2.7 ± 1.0 µm for MOPS, and 1.1 ± 1.3 µm for HI-MOPS. At low ionic strength, FITC-50 permeated into the ESL with a total ESL permeation time (_ESL) of 26 min, and at normal ionic strength with a _ESL of 20 min. No apparent exclusion of FITC-50 from the ESL could be observed at high ionic strength. In conclusion, we demonstrate that the modulation of solvent ionic strength influences the thickness and barrier properties of the ESL.

vascular permeability; isolated artery; ionic strength; glycocalyx

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