HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/3/H1298    most recent 00437.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Mills, W. R. Articles by Laurita, K. R. Search for Related Content PubMed PubMed Citation Articles by Mills, W. R. Articles by Laurita, K. R.

REPORT

Optical mapping of late myocardial infarction in rats

¹Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University; and Departments of ²Cell Biology and ³Cardiovascular Medicine, Cleveland Clinic Foundation, Cleveland, Ohio

Submitted 2 May 2005 ; accepted in final form 28 September 2005

Late myocardial infarction (MI) is associated with ventricular arrhythmias and sudden cardiac death. The exact mechanistic relationship between abnormal cellular electrophysiology, conduction abnormalities, and arrhythmogenesis associated with late MI is not completely understood. We report a novel, rapid dye superfusion technique to enable whole heart, high-resolution optical mapping of late MI. Optical mapping of action potentials was performed in normal rats and rats with anterior MI 7 days after left anterior descending artery ligation. Hearts from normal rats exhibited normal action potentials and impulse conduction. With the use of programmed stimulation to assess arrhythmia inducibility, 29% of hearts with late MI had inducible sustained ventricular tachycardia, compared with 0% in normal rats. A causal relationship between the site of infarction, abnormal action potential conduction (i.e., block and slow conduction), and arrhythmogenesis was observed. Optical mapping techniques can be used to measure high-resolution action potentials in a whole heart model of late MI. This experimental model reproduces many of the electrophysiological characteristics (i.e., conduction slowing, block, and ventricular tachycardia) associated with MI in patients. Importantly, the results of this study can enhance our ability to understand the interplay between cellular heterogeneity, conduction abnormalities, and arrhythmogenesis associated with MI.

arrhythmia; action potential; impulse propagation

This article has been cited by other articles:

				M. Kay, L. Swift, B. Martell, A. Arutunyan, and N. Sarvazyan Locations of ectopic beats coincide with spatial gradients of NADH in a regional model of low-flow reperfusion Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2400 - H2405. [Abstract] [Full Text] [PDF]