HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/5/H2439    most recent 00290.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Padilla, D. J. Articles by Poole, D. C. Search for Related Content PubMed PubMed Citation Articles by Padilla, D. J. Articles by Poole, D. C.

Effects of Type II diabetes on capillary hemodynamics in skeletal muscle

¹Departments of Anatomy and Physiology, and Kinesiology, Kansas State University, Manhattan, Kansas; ²Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas; ³Division of Exercise Physiology, West Virginia University School of Medicine, Morgantown, West Virginia; and ⁴Department of Applied Physics and Chemistry, University of Electro-Communications, Chofu, Tokyo, Japan

Submitted 20 March 2006 ; accepted in final form 5 July 2006

Microcirculatory red blood cell (RBC) hemodynamics are impaired within skeletal muscle of Type I diabetic rats (Kindig CA, Sexton WL, Fedde MR, and Poole DC. Respir Physiol 111: 163–175, 1998). Whether muscle microcirculatory dysfunction occurs in Type II diabetes, the more prevalent form of the disease, is unknown. We hypothesized that Type II diabetes would reduce the proportion of capillaries supporting continuous RBC flow and RBC hemodynamics within the spinotrapezius muscle of the Goto-Kakizaki Type II diabetic rat (GK). With the use of intravital microscopy, muscle capillary diameter (d_c), capillary lineal density, capillary tube hematocrit (Hct_cap), RBC flux (F_RBC), and velocity (V_RBC) were measured in healthy male Wistar (control: n = 5, blood glucose, 105 ± 5 mg/dl) and male GK (n = 7, blood glucose, 263 ± 34 mg/dl) rats under resting conditions. Mean arterial pressure did not differ between groups (P > 0.05). Sarcomere length was set to a physiological length (2.7 µm) to ensure that muscle stretching did not alter capillary hemodynamics; d_c was not different between control and GK rats (P > 0.05), but the percentage of RBC-perfused capillaries (control: 93 ± 3; GK: 66 ± 5 %), Hct_cap, V_RBC, F_RBC, and O₂ delivery per unit of muscle were all decreased in GK rats (P < 0.05). This study indicates that Type II diabetes reduces both convective O₂ delivery and diffusive O₂ transport properties within muscle microcirculation. If these microcirculatory deficits are present during exercise, it may provide a basis for the reduced O₂ exchange characteristic of Type II diabetic patients.

intravital microscopy; capillary network; Goto-Kakizaki rat; spinotrapezius; red blood cell flux

This article has been cited by other articles:

				R. C. Davies, R. G. Eston, D. C. Poole, A. V. Rowlands, F. DiMenna, D. P. Wilkerson, C. Twist, and A. M. Jones Effect of eccentric exercise-induced muscle damage on the dynamics of muscle oxygenation and pulmonary oxygen uptake J Appl Physiol, November 1, 2008; 105(5): 1413 - 1421. [Abstract] [Full Text] [PDF]

				D. C. Poole, M. D. Brown, and O. Hudlicka Counterpoint: There is not capillary recruitment in active skeletal muscle during exercise J Appl Physiol, March 1, 2008; 104(3): 891 - 893. [Full Text] [PDF]

				Rebuttal from Drs. Clark, Rattigan, Barrett, and Vincent J Appl Physiol, March 1, 2008; 104(3): 893 - 893. [Full Text] [PDF]