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PO₂ profiles near arterioles and tissue oxygen consumption in rat mesentery

Department of Physiology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, Virginia

Submitted 18 January 2007 ; accepted in final form 1 May 2007

A scanning phosphorescence quenching microscopy technique, designed to prevent accumulated O₂ consumption by the method, was applied to PO₂ measurements in mesenteric tissue. In an attempt to further increase the accuracy of the measurements, albumin-bound probe was topically applied to the tissue and an objective-mounted pressurized bag was used to reduce the oxygen transport bypass through the thin layer of fluid over the mesentery. PO₂ was measured at multiple sites perpendicular to the blood/wall interface in the vicinity of 84 mesenteric arterioles (7–39 µm in diameter) at distances of 5, 15, 30, 60, 120, and 180 µm in seven anesthetized Sprague-Dawley rats, thereby creating PO₂ profiles. Interstitial PO₂ above and immediately beside arterioles was found to agree with known intravascular values. No significant difference in PO₂ profiles was found between small and large arterioles, indicating a small longitudinal PO₂ gradient in the precapillary mesenteric microvasculature. In addition, the PO₂ profiles were used to calculate oxygen consumption in the mesenteric tissue (56–65 nl O₂·cm^–3·s^–1). Correction of these values for contamination with ambient oxygen yielded an oxygen consumption rate of 60–68 nl O₂·cm^–3·s^–1, the maximal limit for consumption in the mesentery. The results were compared with measurements made by other workers in regard to the employed techniques.

microcirculation; oxygen tension gradients; phosphorescence quenching microscopy

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