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This Article Full Text Full Text (PDF) All Versions of this Article: 297/2/H811    most recent 00385.2009v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Peng, Y. Articles by Penn, M. S. PubMed PubMed Citation Articles by Peng, Y. Articles by Penn, M. S.

Speckle tracking echocardiography in the assessment of mouse models of cardiac dysfunction

Yu Peng,^1,4,* Zoran B. Popovi,^1,* Nikolai Sopko,³ Jeannie Drinko,¹ Zheng Zhang,⁴ James D. Thomas,¹ and Marc S. Penn^1,2,3

¹Department of Cardiovascular Medicine, Heart and Vascular Institute, ²Skirball Laboratory for Cardiovascular Cellular Therapeutics, Center for Cardiovascular Cell Therapy, and ³Department of Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, Cleveland, Ohio; and ⁴Department of Cardiology, The First Affiliated Hospital of Lanzhou University, Lanzhou, China

Submitted 24 April 2009 ; accepted in final form 23 June 2009

Two-dimensional (2-D) speckle tracking echocardiography (STE) accurately quantifies circumferential strain (S_circ) and radial strain (S_rad) in humans and in large and small animals. This study was performed to assess sensitivity of S_circ and S_rad to left ventricular (LV) dysfunction in mouse models. We performed 2-D and M-mode echocardiography 1) in 6 mice during superficial and profound isoflurane anesthesia, 2) serially in 12 mice to monitor the development of heart failure induced by transverse aortic constriction (TAC) and in 8 corresponding control mice, and 3) in 26 mice with varying degrees of TAC-induced heart failure and 12 corresponding control mice immediately before euthanasia. Fractional shortening (FS) and LV mass were measured from standard M-mode tracings, whereas S_circ and S_rad were derived by STE. Percent fibrosis and myocyte diameters were assessed from whole heart cross-sectional specimens stained by Masson trichrome. Profound isoflurane anesthesia decreased S_circ (P = 0.027) but not S_rad (P > 0.05). Mice subjected to TAC showed an immediate and sustained decrease in FS (P = 0.035), S_circ (P = 0.016), and S_rad (P = 0.012). S_circ showed better correlation with FS (r = 0.56 and P < 0.0001) and LV mass (r = 0.42 and P = 0.0003) than S_rad (r = 0.54 and P < 0.0001 for FS and r = 0.37 and P = 0.014 for LV mass, respectively). Percent fibrosis correlated better with S_circ (r = 0.46 and P = 0.004) than with S_rad (r = –0.32 and P = 0.05), whereas myocyte diameter showed similar correlation with both strains (r = 0.45 and r = –0.44, respectively, and P = 0.006 for both). STE correctly identifies LV dysfunction and histological changes in mice and can be used for the serial assessment of cardiac remodeling in murine models.

transverse aortic constriction; systolic strain; systolic function