Hydrogen sulfide (H2S) is a gaseous signaling molecule that appears to be involved in numerous biological processes including regulation of blood pressure and vascular tone. The present study is designed to address the hypothesis that H2S is a functionally significant, endogenous dilator in the newborn cerebrovascular circulation. In vivo experiments were conducted using newborn pigs with surgically implanted, closed, cranial windows. Topical application of H2S concentration-dependently (10-6-2x10-4 M) dilated pial arterioles. This dilation was blocked by glibenclamide (10-6M). L-cysteine, the substrate of the H2S producing enzymes cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), also dilated pial arterioles. The dilation to L-cysteine was blocked by the CSE inhibitor, D,L-propargylglycine (PPG, 10 mM), but was unaffected by the CBS inhibitor, amino-oxyacetate, AOA (1 mM). Western blots detected CSE, but not CBS, in cerebral microvessels, whereas CBS is detected in brain parenchyma. Immunohistological CSE expression is predominantly vascular while CBS is expressed mainly in neurons and astrocytes. L-cysteine (5 mM) increased H2S concentration in CSF, measured by GC/MS, from 561±205 to 2783±818 nM before but not during treatment with PPG (1030±70 to 622±78 nM). Dilation to hypercapnia was inhibited by PPG but not AOA. Hypercapnia increased CSF H2S concentration from 763±243 to 4337±1789 nM before but not during PPG treatment (357±178 versus 425±217 nM). These data show that H2S is a dilator of the newborn cerebral circulation and that endogenous CSE can produce sufficient H2S to decrease vascular tone. H2S appears to be a physiologically significant dilator in the cerebral circulation.
- cerebrovascular circulation
- pial arterioles
- cystathionine γ-lyase
- cystathionine β-synthase
- Copyright © 2010, American Journal of Physiology - Heart and Circulatory Physiology