The incidence of myocardial infarction (MI) and sudden cardiac death increases with age. Here we describe the development of a minimally invasive large animal model of MI that can be applied to young or aged animals. We demonstrate that rabbit coronary anatomy is highly variable, more so than described in previous literature. In this work we categorize the coronary pattern of 37 young and 63 aged rabbits. Aged rabbits had a higher degree of branching from the left main coronary artery. Standardizing the model across age cohorts required a new approach, targeting an area of myocardium rather than a specific vessel. Here we present a method for achieving a reproducible infarct size; one that has yielded a consistent scar encompassing ~30% of the apical, left ventricular free wall. The model consistency has allowed for more valid comparisons of MI sequelae between age cohorts.
- Myocardial Infarction
- Coronary Anatomy
- Coronary Angiography
- Copyright © 2016, American Journal of Physiology-Heart and Circulatory Physiology